PMID: 2489034DOI: 10.1093/intimm/1.4.373Jan 1, 1989Paper
IL-2 receptor subunit, p75: direct demonstration of its IL-2 binding ability by using a novel monoclonal antibody
International Immunology
J SuzukiK Sugamura
Abstract
A new monoclonal antibody (mAb), TU11 mAb, was found to be specific for the p75 subunit of human interleukin 2 receptor (IL-2R). TU11 mAb reacted with human and Gibbon ape cell lines positive for IL-2Rp75 but not with cell lines negative for IL-2Rp75 tested. TU11 mAb specifically detected a single cell surface molecule with a molecular weight of 75 kd. The 75 kd molecule was identical to p75 detected by TU27 mAb specific for human IL-2Rp75 in sequential immunoprecipitation. TU11 mAb did not block IL-2 binding to the high-affinity IL-2R at all and precipitated p75 bound with IL-2. Using TU11 mAb, we have demonstrated here that solubilized p75 has an IL-2 binding site.
Citations
Aug 28, 1991·Journal of Immunological Methods·K OhboK Sugamura
Nov 1, 1995·Brain Research. Brain Research Reviews·U K Hanisch, R Quirion
Aug 1, 2000·Journal of Immunological Methods·H DionyssopoulouG Sotiropoulou
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Mar 3, 2011·The Journal of Biological Chemistry·Yuji AmanoToshikazu Takeshita
Sep 21, 1992·FEBS Letters·S KumakiK Sugamura
Jun 15, 1992·FEBS Letters·H AsaoK Sugamura
Sep 5, 1994·FEBS Letters·H AsaoK Sugamura
Jun 1, 1996·European Journal of Immunology·M HiguchiK Sugamura
Mar 1, 1991·European Journal of Immunology·H DoiT Itoh
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Jun 1, 1996·Journal of Virology·J C MulloyG Franchini
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Jul 17, 1992·Science·T TakeshitaK Sugamura
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