IL-2 receptor subunit, p75: direct demonstration of its IL-2 binding ability by using a novel monoclonal antibody

International Immunology
J SuzukiK Sugamura

Abstract

A new monoclonal antibody (mAb), TU11 mAb, was found to be specific for the p75 subunit of human interleukin 2 receptor (IL-2R). TU11 mAb reacted with human and Gibbon ape cell lines positive for IL-2Rp75 but not with cell lines negative for IL-2Rp75 tested. TU11 mAb specifically detected a single cell surface molecule with a molecular weight of 75 kd. The 75 kd molecule was identical to p75 detected by TU27 mAb specific for human IL-2Rp75 in sequential immunoprecipitation. TU11 mAb did not block IL-2 binding to the high-affinity IL-2R at all and precipitated p75 bound with IL-2. Using TU11 mAb, we have demonstrated here that solubilized p75 has an IL-2 binding site.

Citations

Nov 1, 1995·Brain Research. Brain Research Reviews·U K Hanisch, R Quirion
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S D VossR J Robb
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·N TanakaK Sugamura
Mar 1, 1990·The Journal of Experimental Medicine·H AsaoK Sugamura
Mar 3, 2011·The Journal of Biological Chemistry·Yuji AmanoToshikazu Takeshita
May 1, 2008·Journal of Cell Science·Yuki YamashitaToshikazu Takeshita
Jul 17, 1992·Science·T TakeshitaK Sugamura

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