IL-22-Expressing Murine Lymphocytes Display Plasticity and Pathogenicity in Reporter Mice

Frontiers in Immunology
Wei ShenScott K Durum

Abstract

IL-22 has multiple activities ranging from tissue repair to inflammation. To characterize the pathogenicity and plasticity of cells that produce IL-22, a novel reporter mouse strain was generated. Homeostatic IL-22 reporter expression was observed in intestinal lymphoid cells identified as CD4 T cells and ILC3 cells. In a model of inflammatory bowel disease, CD4 T cells strongly expressed the IL-22 reporter in mesenteric lymph node. To examine plasticity of IL-22(+) T cells, they were purified after generation in vitro or in vivo from inflamed colon, and then cultured under Th1, Th2, or Th17 conditions. In vitro-generated IL-22(+) CD4 T cells showed relatively durable IL-22 expression under Th1 or Th2 conditions, whereas in vivo-generated cells rapidly lost IL-22 expression under these conditions. In vitro-generated cells could not be diverted to express Th1 or Th2 cytokines despite the expression of "master regulators." In vivo-generated cells could be diverted, at very low frequency, to express Th1 or Th2 cytokines. Both in vitro- and in vivo-generated cells could be induced in vitro to express high levels of IL-17A and IL-17F, assigning them to a "Th17 biased" class. However, IL-27 potently downregulated IL-22 expression. To...Continue Reading

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Citations

Jun 16, 2016·Frontiers in Immunology·Silvia Gimeno BriasIan R Humphreys
Jan 20, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maximilian W PlankPaul S Foster
Jul 28, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adam K SavageRichard M Locksley

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Methods Mentioned

BETA
FACS
flow cytometry
electrophoresis
PCR
PMA
Profiler

Software Mentioned

FACS Express
ImageJ
FlowJo
GraphPad Prism

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