IL-24 inhibits lung cancer cell migration and invasion by disrupting the SDF-1/CXCR4 signaling axis.

PloS One
Janani PanneerselvamRajagopal Ramesh

Abstract

The stromal cell derived factor (SDF)-1/chemokine receptor (CXCR)-4 signaling pathway plays a key role in lung cancer metastasis and is molecular target for therapy. In the present study we investigated whether interleukin (IL)-24 can inhibit the SDF-1/CXCR4 axis and suppress lung cancer cell migration and invasion in vitro. Further, the efficacy of IL-24 in combination with CXCR4 antagonists was investigated. Human H1299, A549, H460 and HCC827 lung cancer cell lines were used in the present study. The H1299 lung cancer cell line was stably transfected with doxycycline-inducible plasmid expression vector carrying the human IL-24 cDNA and used in the present study to determine the inhibitory effects of IL-24 on SDF-1/CXCR4 axis. H1299 and A549 cell lines were used in transient transfection studies. The inhibitory effects of IL-24 on SDF1/CXCR4 and its downstream targets were analyzed by quantitative RT-PCR, western blot, luciferase reporter assay, flow cytometry and immunocytochemistry. Functional studies included cell migration and invasion assays. Endogenous CXCR4 protein expression levels varied among the four human lung cancer cell lines. Doxycycline-induced IL-24 expression in the H1299-IL24 cell line resulted in reduced CX...Continue Reading

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Citations

Apr 16, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhidong WangYong Zhou
Jun 23, 2016·Journal of Nanobiotechnology·Ranganayaki MuralidharanRajagopal Ramesh
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Nov 1, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Jun ShaoMing-Qing Li
Sep 8, 2016·Oncotarget·Janani PanneerselvamRajagopal Ramesh
May 17, 2019·Molecular Pharmacology·Joyce HeuninckCarsten Hoffmann
May 27, 2015·Oncotarget·Janani PanneerselvamRajagopal Ramesh
Sep 7, 2017·Current Medicinal Chemistry·Weiqiang ZhouGuangdi Wang
May 10, 2019·Oncoimmunology·Xiaolin WangWenling Han
Dec 1, 2019·Cancers·Janani PanneerselvamRajagopal Ramesh
Apr 24, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jonathan M CiencewickiSteven R Kleeberger
Sep 26, 2020·Life Sciences·Devivasha BordoloiAjaikuamr B Kunnumakkara
Feb 11, 2021·International Journal of Molecular Sciences·Shu-Chun ChangJeak Ling Ding
Feb 14, 2020·International Journal of Radiation Biology·Meghna MehtaAnupama Munshi

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Methods Mentioned

BETA
transfection
antisense oligonucleotide
Flow cytometry

Software Mentioned

Image Quant

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