PMID: 25726525Mar 3, 2015Paper

IL-32θ inhibits monocytic differentiation of leukemia cells by attenuating expression of transcription factor PU.1

Oncotarget
Man Sub KimDo-Young Yoon

Abstract

PU.1 is a key transcription factor regulating the myeloid differentiation. PU.1-induced monocytic differentiation into macrophage is also important for blood cancer development. Therefore, we chose THP-1 monocytic leukemia cells to investigate the function of a recently discovered IL-32θ. Genetic analyses identified differences in the sequences of IL-32θ and IL-32β. Using previously established cell lines that stably express IL-32θ and IL-32β and cell lines transiently expressing IL-32θ, we observed that expression of IL-32θ inhibited phorbol 12-myristate 13-acetate (PMA)-induced monocytic differentiation in both THP-1 and HL-60 cells. IL-32θ also suppressed expression of the macrophage cell surface markers, CD11b, CD18, and CD36. Interestingly, expression of IL-32β or IL-32θ had no effect on the expression levels of cell cycle related factors. As a result, we concluded that these isoforms did not contribute to PMA-induced cell cycle arrest. IL-32θ was found to modulate expression of PU.1, a transcription factor necessary for myeloid lineage commitment. Transient expression of PU.1 in THP-1/IL-32θ cells rescued the observed differentiation defect. Additionally, transient expression of both CCAAT-enhancer-binding protein α (C/EB...Continue Reading

References

Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·A G RosmarinC Simkevich
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·P ZhangZ Sun
Nov 5, 1999·Protein Science : a Publication of the Protein Society·F PioR A Maki
Mar 16, 2005·Molecular and Cellular Biology·Yutaka OkunoDaniel G Tenen
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Leo A B JoostenWim B van den Berg
Jan 4, 2007·The Journal of Clinical Investigation·Filip K SwirskiMikael J Pittet
Jan 30, 2007·Nature Reviews. Immunology·Frank Rosenbauer, Daniel G Tenen
Jun 22, 2007·Immunity·Hiromi Iwasaki, Koichi Akashi
Feb 7, 2008·Journal of Immunological Methods·Ki-Hong KimDo-Young Yoon
Sep 6, 2008·Immunology·Ji-Da ChoiSoo-Hyun Kim
Jan 10, 2009·Annual Review of Immunology·Cedric AuffrayFrederic Geissmann
Jan 13, 2009·PloS One·Guillaume Mabilleau, Afsie Sabokbar
Feb 18, 2009·Blood·Daniel NowakH Phillip Koeffler
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Bas HeinhuisLeo A B Joosten
May 10, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mi Young JungTae Sung Kim
Oct 26, 2011·Nature Reviews. Immunology·Andrew ChowMiriam Merad
Aug 15, 2012·Infection and Immunity·Kosuke SakitaniKazuhiko Koike
Mar 5, 2013·Oncotarget·Yutaka Okuno, Hiromichi Yuki
Mar 13, 2013·Medical Oncology·Sumiya IshigamiShoji Natsugoe
Mar 26, 2013·Cellular and Molecular Life Sciences : CMLS·René HuberKorbinian Brand
Jan 30, 2014·Biochimie·Jeong-Woo KangDo-Young Yoon

❮ Previous
Next ❯

Citations

May 28, 2019·Cell Communication and Signaling : CCS·Thu-Huyen PhamDo-Young Yoon
Sep 2, 2020·Journal of Leukocyte Biology·Kristin Roseth AassTherese Standal
Dec 19, 2020·Scandinavian Journal of Immunology·Rafael de AlbuquerqueRiitta Lahesmaa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.