IL-35Ig-expressing dendritic cells induce tolerance via Arginase 1

Journal of Cellular and Molecular Medicine
Eleonora PanfiliMaria Laura Belladonna

Abstract

The cytokine interleukin IL-35 is known to exert strong immunosuppressive functions. Indoleamine 2,3-dioxygenase 1 (IDO1) and Arginase 1 (Arg1) are metabolic enzymes that, expressed by dendritic cells (DCs), contribute to immunoregulation. Here, we explored any possible link between IL-35 and the activity of those enzymes. We transfected a single chain IL-35Ig gene construct in murine splenic DCs (DC35 ) and assessed any IDO1 and Arg1 activities as resulting from ectopic IL-35Ig expression, both in vitro and in vivo. Unlike Ido1, Arg1 expression was induced in vitro in DC35 , and it conferred an immunosuppressive phenotype on those cells, as revealed by a delayed-type hypersensitivity assay. Moreover, the in vivo onset of a tolerogenic phenotype in DC35 was associated with the detection of CD25+ CD39+ , rather than Foxp3+ , regulatory T cells. Therefore, Arg1, but not Ido1, expression in DC35 appears to be an early event in IL-35Ig-mediated immunosuppression.

References

Jun 1, 1994·European Journal of Immunology·P PuccettiU Grohmann
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·U GrohmannP Puccetti
May 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Francesca FallarinoPaolo Puccetti
Jan 24, 2009·Trends in Molecular Medicine·Maria L BelladonnaPaolo Puccetti
Aug 20, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joshua D MezrichChristopher A Bradfield
Mar 5, 2013·The Journal of Clinical Investigation·David Vasquez-DunddelYoung Kim
Apr 20, 2014·Nature Medicine·Ren-Xi WangCharles E Egwuagu
Jan 20, 2016·Nature Immunology·Peter J Murray
Jul 12, 2016·Nature Reviews. Immunology·Luke A J O'NeillJeff Rathmell
May 30, 2017·Current Opinion in Pharmacology·Giada MondanelliVincenzo Bronte
Jan 30, 2018·Frontiers in Immunology·Matthias P DomogallaKerstin Steinbrink
Mar 27, 2018·International Journal of Cancer. Journal International Du Cancer·Manouchehr TeymouriAmirhosein Sahebkar
May 8, 2018·Autoimmunity Reviews·Lin-Chong SuWang-Dong Xu

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Citations

Jun 6, 2020·Frontiers in Immunology·Tomasz M GrzywaJakub Golab
Jun 8, 2021·Frontiers in Immunology·Kewei LiuKe Jiang

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Methods Mentioned

BETA
transfection
PCR
ELISA
flow cytometry

Software Mentioned

FlowJo

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