Abstract
The colon carcinoma cell line HT-29 was used to explore the potential of interleukin-4 (IL-4) and tumor necrosis factor alpha (TNF-alpha) to modify integrin expression and adhesive functions of tumor cells in vitro and to examine corresponding metastatic effects in vivo. Preincubation of HT-29 cells with 100 U/ml of IL-4 for 48 h downregulated the surface expression of the integrin subunits alpha 2, alpha 3, beta 1 and beta 4 after 48 h, whereas the alpha 1 subunit was upregulated. In contrast, 100 U/ml to TNF-alpha selectively upmodulated the expression of alpha v. Attachment to fibronectin of cells treated with IL-4 increased twofold (63.5% vs 32.4%). Adhesion to fibronectin (54.0% vs 32.4%) and vitronectin (37.9% vs 16.4%) was elevated in the case of TNF-alpha stimulation. Using an experimental metastasis model, HT-29 cells showed a significant reduction of their lung-colonizing potential in nude mice when preincubated with IL-4 for 48 h before intravenous injection. The decrease also observed for TNF-alpha-treated cells was less pronounced. The data indicate that the cytokines IL-4 and TNF-alpha can act as direct regulators of adhesive mechanisms of tumor cells bearing adequate receptors, thus influencing lung-colony format...Continue Reading
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R E SeftorM J Hendrix
Oct 1, 1992·British Journal of Cancer·L KaklamanisA L Harris
Oct 1, 1992·Journal of Cellular Physiology·C T BassonJ A Madri
May 11, 1992·The Journal of Cell Biology·E C LeeA M Mercurio
Apr 3, 1992·Cell·R O Hynes
May 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A MantovaniE Dejana
Nov 1, 1991·The Journal of Pathology·A J D'ArdenneS Jordan
Feb 1, 1992·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S A Rosenberg
Aug 1, 1991·The Journal of Cell Biology·P DefilippiG Tarone
Jan 16, 1991·Journal of the National Cancer Institute·M R BaniR Giavazzi
Feb 1, 1991·International Journal of Cancer. Journal International Du Cancer·J VaraniS Chakrabarty
Feb 20, 1991·International Journal of Cancer. Journal International Du Cancer·R MortariniG Parmiani
Mar 9, 1990·Cell·F G Giancotti, E Ruoslahti
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U M WewerR Albrechtsen
Mar 1, 1988·The Journal of Cell Biology·K R GehlsenE Ruoslahti
Feb 1, 1989·Journal of Cellular Physiology·S Dedhar
Feb 1, 1989·British Journal of Cancer·I SaikiI Azuma
Feb 1, 1986·The Journal of Cell Biology·R B RunyanB D Shur
Nov 1, 1993·Acta Pathologica Japonica·K ArihiroT Toge
Jul 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R SagerG Sotiropoulou
Sep 30, 1993·International Journal of Cancer. Journal International Du Cancer·C L SchreinerR L Juliano
Jun 1, 1992·Trends in Cell Biology·F Van Roy, M Mareel
Citations
Sep 19, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T Meyer, I R Hart
May 16, 2000·Immunology·C MacíasP Hernández
May 9, 2009·Head & Neck·Marcos Vinícius Macedo de OliveiraAlfredo Maurício Batista D Paula
Jul 10, 1997·Experimental Cell Research·H KempermanE Roos
Apr 9, 2015·Reproductive Sciences·Federica QuattronePaola Panina-Bordignon
Aug 22, 2009·Nature Reviews. Immunology·Grant McFaddenEric Bartee
Sep 2, 1999·British Journal of Cancer·J HaierG L Nicolson
Aug 25, 2016·Oncotarget·Jason R ToddPaul H Huang
Oct 31, 2000·Microscopy Research and Technique·J StutzmannP Simon-Assmann
Feb 27, 1999·International Journal of Cancer. Journal International Du Cancer·R VogelmannM P Lutz
Jan 17, 2021·International Journal of Molecular Sciences·Xujun SongMarko Kornmann
Nov 5, 1997·Biochemical and Biophysical Research Communications·M SuzukiM Sokabe