PMID: 8605730Mar 1, 1996Paper

IL-4 and TNF-alpha induce changes in integrin expression and adhesive properties and decrease the lung-colonizing potential of HT-29 colon carcinoma cells

Clinical & Experimental Metastasis
F HerzbergE D Kreuser

Abstract

The colon carcinoma cell line HT-29 was used to explore the potential of interleukin-4 (IL-4) and tumor necrosis factor alpha (TNF-alpha) to modify integrin expression and adhesive functions of tumor cells in vitro and to examine corresponding metastatic effects in vivo. Preincubation of HT-29 cells with 100 U/ml of IL-4 for 48 h downregulated the surface expression of the integrin subunits alpha 2, alpha 3, beta 1 and beta 4 after 48 h, whereas the alpha 1 subunit was upregulated. In contrast, 100 U/ml to TNF-alpha selectively upmodulated the expression of alpha v. Attachment to fibronectin of cells treated with IL-4 increased twofold (63.5% vs 32.4%). Adhesion to fibronectin (54.0% vs 32.4%) and vitronectin (37.9% vs 16.4%) was elevated in the case of TNF-alpha stimulation. Using an experimental metastasis model, HT-29 cells showed a significant reduction of their lung-colonizing potential in nude mice when preincubated with IL-4 for 48 h before intravenous injection. The decrease also observed for TNF-alpha-treated cells was less pronounced. The data indicate that the cytokines IL-4 and TNF-alpha can act as direct regulators of adhesive mechanisms of tumor cells bearing adequate receptors, thus influencing lung-colony format...Continue Reading

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Citations

Sep 19, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T Meyer, I R Hart
Apr 9, 2015·Reproductive Sciences·Federica QuattronePaola Panina-Bordignon
Aug 22, 2009·Nature Reviews. Immunology·Grant McFaddenEric Bartee
Oct 31, 2000·Microscopy Research and Technique·J StutzmannP Simon-Assmann
Feb 27, 1999·International Journal of Cancer. Journal International Du Cancer·R VogelmannM P Lutz
Jan 17, 2021·International Journal of Molecular Sciences·Xujun SongMarko Kornmann
Nov 5, 1997·Biochemical and Biophysical Research Communications·M SuzukiM Sokabe

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