PMID: 7547683Apr 1, 1995Paper

IL-4-induced B cell migration involves transient interactions between beta 1 integrins and extracellular matrix components

International Immunology
C Elenström-MagnussonE Severison

Abstract

IL-4 has previously been shown to stimulate motile responses in murine B lymphocytes. This was studied as acquisition of motile morphology and migration through filters in microchemotaxis chambers. In this paper, we investigated IL-4-stimulated migration of B cells into gels of native collagen fibers, which may be a more physiologically relevant assay. When IL-4 was present in the gel and/or in the medium above, B cells were able to invade the collagen gel. Migration was dependent on the dose of IL-4 and was optimal after 45 h of incubation. It appeared that IL-4 acted by inducing both chemokinesis and chemotaxis. Fibronectin (FN) was found to be an important factor for B cell locomotion, since low concentrations of FCS or FN in the gel matrix greatly improved migration. B cell locomotion was inhibited by antibodies specific for beta 1, alpha 4 and alpha 5 integrins, indicating the presence of integrin-extracellular matrix (ECM) interactions in lymphocyte motility responses. Migration was not associated with an up-regulation of beta 1, alpha 4 or alpha 5 integrins. The adhesion between substrate and cells is likely to be of low affinity, since IL-4-stimulated, as well as non-stimulated B cells, did not adhere to ECM-coated cult...Continue Reading

Citations

Sep 3, 2010·Journal of Mammary Gland Biology and Neoplasia·Ori MallerPepper Schedin
Nov 8, 1996·Journal of Chromatography. B, Biomedical Applications·J DucharmeR Farinotti
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·M J Tino, J R Wright
Jul 19, 2003·Scandinavian Journal of Immunology·E Severinson, L Westerberg
Aug 16, 2019·International Journal of Molecular Sciences·Barbara Świerczek-LasekKarolina Archacka
Jun 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michelle J MuesselAndrew J Wardlaw
Feb 10, 2006·Experimental Cell Research·J F LafreniereJ P Tremblay

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