IL-4 modulates transcriptional control of the mannose receptor in mouse FSDC dendritic cells

Archives of Biochemistry and Biophysics
Brian S EganV L Shepherd

Abstract

The mannose receptor is a 175 kDa protein found on the surface of macrophages and dendritic cells whose functions include clearance of extracellular hydrolases, internalization of pathogens, and antigen capture. Receptor expression is closely linked to the functional state of these cells and is regulated by cytokines. Previous work has shown that treatment of macrophages and dendritic cells with interleukin-4 leads to increased mannose receptor expression. We have examined the mechanism of this IL-4-mediated up-regulation in the murine dendritic cell line FSDC. IL-4 increased mannose receptor activity, protein, and mRNA. The mannose receptor promoter was functional in FSDCs using transient transfection assays, and IL-4 treatment increased promoter activity 2.6-fold. The responsive region was localized to the proximal 228 bp. Electrophoretic mobility shift assays detected an IL-4-inducible protein that bound to the mannose receptor promoter at a site spanning the region between -147 and -108 bp. The sequence TTAC(N)4CACC (-135 and -124 bp) is similar to the IL-4 response region in the Fc receptor II. Mutation of the flanking TT and CC in this motif blocked IL-4 responsiveness and binding of the IL-4-induced mannose receptor bind...Continue Reading

References

Jul 1, 1992·Archives of Biochemistry and Biophysics·H B CowanV L Shepherd
Aug 1, 1995·European Journal of Immunology·G GirolomoniP Ricciardi-Castagnoli
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·H M SeidelJ Rosen
Aug 1, 1994·Archives of Biochemistry and Biophysics·V L ShepherdS Vick
Mar 1, 1996·European Journal of Immunology·M B LutzP Ricciardi-Castagnoli
Jun 1, 1996·Current Opinion in Immunology·A Lanzavecchia
Oct 1, 1996·Molecular and Cellular Biology·T MikitaU Schindler
Apr 2, 1998·Current Opinion in Immunology·P D Stahl, R A Ezekowitz
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Nov 13, 1998·The Journal of Biological Chemistry·A LischkeK Friedrich
Nov 20, 1998·Scandinavian Journal of Immunology·S R Paludan
Mar 20, 1999·The Journal of Biological Chemistry·B S EganV L Shepherd

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Citations

Sep 13, 2012·BMC Immunology·David J VigerustVirginia L Shepherd
Mar 9, 2012·Biomaterials·Keerti JainNarendra K Jain
Dec 14, 2017·Scientific Reports·Svenja F B MennensKoen van den Dries
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Els den DekkerRuurd Torensma

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