IL-4 mRNA transcription is induced in mouse bone marrow-derived mast cells through an MHC class II-dependent signaling pathway

European Journal of Immunology
P FrandjiS Mécheri

Abstract

We have previously shown that mouse bone marrow-derived mast cells (BMMC) can process and present immunogenic peptides to CD4 T cells. Here, we report on a T cell-dependent MHC class II-mediated mast cell activation resulting in IL-4 transcription and protein release. Presentation of optimal doses of ovalbumin peptide 323-339 resulted in IL-2 production by a specific T cell hybridoma and increase in IL-4 mRNA transcription in mast cells. IL-4 mRNA transcription increased by 200-fold in mast cells treated in IL-3/IL-4/granulocyte-macrophage colony-stimulating factor (high presenters) whereas only a tenfold increase or no increase were obtained with IL-3/IL-4/IFN-gamma- or IL-3-treated mast cells (low presenters), respectively. Induction of IL-4 mRNA transcription in purified mast cells by direct ligation of MHC class II molecules, using anti-I-A and anti-I-E-coated beads, indicates that MHC class II molecules are critical in this signaling pathway. However, when compared to T cells, anti-MHC class II-coated beads were less efficient, indicating a potential role of accessory molecules in this mast cell activation process. IgE-independent IL-4 production by mast cells as a result of cognate interaction with CD4 T cells could be cr...Continue Reading

References

Jan 1, 1977·Journal of Immunological Methods·S B TuckerR E Jordon
Dec 1, 1991·Current Opinion in Immunology·S J GalliB K Wershil
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E RazinR A Good
Jan 1, 1983·Annual Review of Pharmacology and Toxicology·D LagunoffG Read
Aug 1, 1983·The Journal of Experimental Medicine·R ShimonkevitzH Grey
Sep 1, 1994·Immunology Today·P R Scholl, R S Geha
Apr 1, 1995·The Journal of Experimental Medicine·P R BurdF C Mills
Jan 28, 1993·The New England Journal of Medicine·S J Galli

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Citations

Aug 19, 1999·Critical Reviews in Oncology/hematology·P Bradding, S T Holgate
Nov 22, 2008·Avian Pathology : Journal of the W.V.P.A·Quan SunYe Ding
Apr 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susumu NakaeStephen J Galli
Feb 18, 2010·Indian Journal of Dermatology·C L Goh, K T Tan
Feb 28, 2002·International Immunology·Zhonglin WuDavid B G Oliveira
Feb 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susumu NakaeStephen J Galli
Nov 23, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J M BrownD D Metcalfe
May 15, 2007·Immunological Reviews·Blayne Amir Sayed, Melissa A Brown
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G CaiC A Hunter
Dec 21, 2007·Clinical Reviews in Allergy & Immunology·Malcolm W Greaves, Kian Teo Tan
Jun 22, 2002·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Z Z ZhaoL J Walsh
Feb 13, 2001·Experimental Dermatology·B M HenzM Babina

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