IL-6 blockade preferentially inhibits Th17 differentiation in collagen-induced arthritis.

Rheumatology International
Hiroto YoshidaMasahiko Mihara

Abstract

We examined the time course of cytokine production by CD4 T cells from mice with collagen-induced arthritis (CIA), and we determined the influence of interleukin-6 (IL-6) blockade on cytokine production. CD4 T cells purified from spleen were cultured with both collagen and anti-CD28 antibody for 48 h and the production of interferon-γ (IFN-γ), IL-4, and IL-17 by the cells, secreted into the supernatants, was measured at various time intervals. The production of all these cytokines started 7 days after the first immunization. A marked increase in IFN-γ production was observed after the second immunization, but IL-4 and IL-17 production was not affected by a second immunization. A single injection of anti-mouse IL-6 receptor antibody (MR16-1) on the day of the first immunization suppressed the onset of arthritis. IL-17 production by CD4 T cells from MR16-1-treated mice was significantly lower than that from the control mice. On the other hand, treatment with MR16-1 showed only a tendency to suppress the production of IL-4 and IFN-γ. Injection of MR16-1 on day 21 did not suppress the onset of arthritis. We examined the direct influence of MR16-1 on cytokine production by differentiated CD4 T cells from arthritic mice. Production o...Continue Reading

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Citations

Apr 1, 2013·Clinical & Translational Immunology·Steven O'ReillyJacob M van Laar
Jun 25, 2015·Immunological Investigations·Mansheng LuoJimin Gao
Apr 12, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B M BuchholzA J Bauer
Nov 18, 2011·Nanomedicine·Robert I ScheinmanUday B Kompella
Jul 7, 2021·Autoimmunity Reviews·Tsutomu TakeuchiSakae Tanaka

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