IL-7 prevents both caspase-dependent and -independent pathways that lead to the spontaneous apoptosis of i-IEL

Cellular Immunology
S YadaT Lin

Abstract

Intestinal intraepithelial lymphocytes (i-IEL) readily undergo spontaneous apoptosis in vitro through an unclear mechanism. Here we examined the relationship between caspases, which plays a major role in apoptosis, and IL-7 in the spontaneous apoptosis of i-IEL in vitro. We demonstrated that IL-7 and zVAD prevented the spontaneous apoptosis of i-IEL by approximately 50% and 25% respectively with no additive protection seen when both are used. IL-7 preferentially prevented the apoptosis of gammadelta i-IEL, while zVAD equally prevented the apoptosis of gammadelta and alphabeta i-IEL. Lastly, we demonstrated that the spontaneous apoptosis of i-IEL is associated with a marked increase in caspase activity. Caspase activity was completely inhibited by zVAD, but only slightly by IL-7. Overall these results suggest that two pathways lead to the spontaneous apoptosis of i-IEL, one which is caspase dependent and the other which is caspase independent. IL-7 appears to exert its effect on i-IEL undergoing spontaneous by partially inhibiting both apoptotic pathways.

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Citations

Apr 12, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hua YangDaniel H Teitelbaum
Jan 12, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hua YangDaniel H Teitelbaum
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Valerie I BrownStephan A Grupp
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May 22, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Francisco León, Garbiñe Roy
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May 11, 2004·Journal of Applied Physiology·L Hoffman-GoetzJ Guan

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