IL-8 Enhances Therapeutic Effects of BMSCs on Bone Regeneration via CXCR2-Mediated PI3k/Akt Signaling Pathway

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Aijun YangJianzhong Xu

Abstract

Tissue engineering bone transplantation with bone marrow mesenchymal stem cells (BMSCs) is an effective technology to treat massive bone loss, while molecular regulation of the bone regeneration processes remains poorly understood. Here, we aimed to assess the role of interleukin-8 (IL-8) in the recruitment of host cells by seeded BMSCs and in the bone regeneration. A transwell assay was performed to examine the role of IL-8/CXCR1/CXCR2/PI3k/Akt on the migration potential of hBMSCs. The in vitro chondrogenic differentiation of hBMSCs was assessed by examination of 2 chondrogenic markers, Sox9 and type 2 collagen (COL2). mBMSCs were used in tissue engineered bone (TEB) with/without IL-8 implanted into bone defect area with CXCR2 or Akt inhibitors. Density and Masson staining of the regenerated bone were assessed. The chondrogenesis was assessed by expression levels of associated proteins, Sox9 and COL2, by RT-qPCR and by immunohistochemistry. IL-8 may trigger in vitro migration of hBMSCs via CXCR2-mediated PI3k/Akt signaling pathway. IL-8 enhances osteogenesis in the TEB-implanted bone defect in mice. IL-8 induces chondrogenic differentiation of hBMSCs via CXCR2-mediated PI3k/Akt signaling pathway in vitro and in vivo. IL-8 enha...Continue Reading

Citations

Feb 28, 2020·Experimental and Therapeutic Medicine·Su LiuJianfei Ge
Mar 3, 2020·Frontiers in Immunology·Deniz RagipogluVerena Fischer
Sep 26, 2019·Frontiers in Cardiovascular Medicine·Faez Saleh Al-HamedFaleh Tamimi
Feb 9, 2019·Tissue Engineering. Part C, Methods·Junchao XingTianyong Hou
Feb 5, 2021·Stem Cell Research & Therapy·Nurul Dinah KadirEng Hin Lee
Mar 7, 2021·International Journal of Molecular Sciences·Sarah Al-MaawiShahram Ghanaati
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Olena RakhimovaNelly Romani Vestman

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