PMID: 8604020Mar 1, 1996Paper

IL-8 induces T cell chemotaxis, suppresses IL-4, and up-regulates IL-8 production by CD4+ T cells

Journal of Leukocyte Biology
B GesserC G Larsen

Abstract

Interleukin-8 (IL-8), a neutrophil-activating cytokine, also activates certain T cell functions such as chemotaxis. We additionally find (n = 6) that recombinant (rIL-8; 1-100 ng/ml), when added to 24 h culture of human CD4+ T cells, suppressed the spontaneous production of IL-4 (50-85%). Steady state production of Il-4 was typically around 30 pg/ml, determined by use of a solid- phase immunoabsorbant assay. De novo synthesis of IL-4 from CD4+ T cells cultured for 3 days was also evaluated by use of detection of [35S]methionine incorporation, as visualized by autoradiography of 2-D gels, and showed that IL-8 suppressed IL-4 production. This suppression of IL-4 production was confirmed in the cytosol fraction by use of Western blotting. The effect of IL-8 (100 ng/ml) was comparable to that of 10 ng/ml recombinant interferon-gamma, both strongly suppressing IL-4 production. The regulatory effect of IL-8 on IL-4 production was also indicated by the fact that addition of a neutralizing monoclonal anti-IL-8 antibody (WS.4) enhanced the spontaneous IL-4 production when added to the culture of CD4+ T cells, thereby probably inactivating the effect of IL-8 originating from the cultured T cells. Also, we observed that IL-4 mRNA expressi...Continue Reading

Citations

Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·B GesserC G Larsen
Nov 15, 2000·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·S PaltrinieriP Sartorelli
Aug 19, 2011·Journal of Receptor and Signal Transduction Research·Indranil MukhopadhyayNeelima Khairatkar-Joshi
Nov 30, 2016·Veterinary Research·Nitish BoodhooShahriar Behboudi
Dec 11, 2002·Experimental Dermatology·Y TokuraS Moriwaki
Apr 7, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Richard S BlumbergSean P Colgan
Mar 30, 2002·Nature Biotechnology·Barry SchweitzerStephen F Kingsmore
Mar 17, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nadia MorettoRiccardo Patacchini
Jul 15, 2011·Clinical and Vaccine Immunology : CVI·Eva SonckEric Cox
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·C E BelcherD A Relman
Jul 30, 2015·The Cancer Journal·Tiziana CotechiniLisa M Coussens
Dec 14, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R ArnoldW König
Nov 19, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andreas LundqvistRichard W Childs
Sep 18, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nadia MorettoFabrizio Facchinetti
Jan 2, 2021·International Journal of Molecular Sciences·Claudia CurciFabio Sallustio
Jul 15, 2021·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Stavros TryfonGeorge Ismailos

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