IL15 induces a potent antitumor activity in NK cells isolated from malignant pleural effusions and overcomes the inhibitory effect of pleural fluid

Oncoimmunology
D CroxattoP Vacca

Abstract

Natural Killer (NK) cells are capable of recognizing and killing cancer cells and play an important role in tumor immunosurveillance. However, tumor-infiltrating NK cells are frequently impaired in their functional capability. A remarkable exception is represented by NK cells isolated from malignant pleural effusions (PE) that are not anergic and, upon IL2-induced activation, efficiently kill tumor cells. Although IL2 is used in various clinical trials, severe side effects may occur in treated patients. In this study, we investigated whether also other clinical-grade cytokines could induce strong cytotoxicity in NK cells isolated from pleural fluid of patients with primary or metastatic tumors of different origins. We show that PE-NK cells, cultured for short-time intervals with IL15, maintain the CD56bright phenotype, a high expression of the main activating receptors, produce cytokines and kill tumor cells in vitro similarly to those treated with IL2. Moreover, IL15-activated PE-NK cells could greatly reduce the growth of established tumors in mice. This in vivo antitumor effect correlated with the ability of IL15-activated PE-NK cells to traffic from periphery to the tumor site. Finally, we show that IL15 can counteract the ...Continue Reading

References

Jan 1, 1996·Annual Review of Immunology·A MorettaL Moretta
Jun 1, 2001·The Journal of Thoracic and Cardiovascular Surgery·I TakanamiM Giga
Nov 8, 2001·Trends in Immunology·M A CooperM A Caligiuri
Dec 26, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Francisco R VillegasLuis Callol
Jun 19, 2002·The Journal of Experimental Medicine·Todd C BeckerRafi Ahmed
Aug 13, 2003·The Journal of Experimental Medicine·Cristina BottinoAlessandro Moretta
Nov 9, 2004·Trends in Immunology·Lorenzo MorettaAlessandro Moretta
Mar 18, 2005·Annual Review of Immunology·Lewis L Lanier
May 12, 2005·Cancer Immunology, Immunotherapy : CII·Jose Manuel CózarFrancisco Ruiz-Cabello Osuna
Sep 28, 2005·The Journal of Experimental Medicine·François GhiringhelliLaurence Zitvogel
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chiara RomagnaniGuido Ferlazzo
Jul 25, 2008·Blood·Michael A Caligiuri
Feb 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Megan A CooperWayne M Yokoyama
Jun 17, 2009·The Journal of Experimental Medicine·Cameron S BrandtSteven D Levin
Jul 2, 2009·Neoplasia : an International Journal for Oncology Research·Giuseppe SconocchiaLuigi Tornillo
Jan 12, 2010·American Journal of Hematology·Dahlia ElkadiTommy R Tong
Jul 27, 2010·Trends in Immunology·Shannon BurkeEnnio Carbone
Aug 4, 2010·Cancer Immunology, Immunotherapy : CII·Veronika BachanovaJeffrey S Miller
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrea De MariaLorenzo Moretta
Jan 8, 2011·Science·Eric VivierSophie Ugolini
Jan 12, 2011·Annual Review of Immunology·Matthew D VeselyMark J Smyth
Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Niels HalamaChristine S Falk
May 3, 2011·Cytokine & Growth Factor Reviews·Marek JakobisiakWitold Lasek
Aug 16, 2011·The Journal of Clinical Investigation·Emilie MamessierDaniel Olive
Aug 17, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maria R ParkhurstSteven A Rosenberg
Aug 19, 2011·Cancer Research·Wolf Herman FridmanGuido Kroemer
Aug 27, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Judith EcklElfriede Noessner
Mar 16, 2012·Nature Reviews. Cancer·Wolf Herman FridmanJérôme Galon
Dec 5, 2012·The Journal of Experimental Medicine·Jing NiAdelheid Cerwenka
Jan 15, 2013·Frontiers in Immunology·Stephanie Krieg, Evelyn Ullrich
Jan 17, 2013·European Journal of Immunology·Magali TermeEric Tartour
Jan 26, 2013·Nature Reviews. Immunology·Hergen SpitsEric Vivier
Aug 21, 2013·Blood·Florence BaychelierVincent Vieillard
Jan 18, 2014·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Jeffrey W LeongTodd A Fehniger

❮ Previous
Next ❯

Citations

Mar 12, 2019·International Journal of Cancer. Journal International Du Cancer·Nicola TuminoLorenzo Moretta
Nov 5, 2019·Journal of Cellular Biochemistry·S Haleh HosseiniHamed Mohammadi
Apr 27, 2020·British Journal of Pharmacology·F R MariottiL Moretta
Mar 25, 2019·Oncoimmunology·Pranav MurthyMichael T Lotze
Feb 6, 2020·Frontiers in Immunology·Paola VaccaLorenzo Moretta
Oct 6, 2020·Frontiers in Immunology·Adam C SoloffMichael T Lotze

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
biopsy
flow cytometry
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad Prism
FlowJo

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.