IL2rg Cytokines Enhance Umbilical Cord Blood CD34+ Cells Differentiation to T Cells

Advanced Pharmaceutical Bulletin
Z AliyariH Nozad Charoudeh

Abstract

Umbilical cord blood (UCB) is an alternative source of hematopoietic stem cell (HSC) transplantation for the treatment of patients with leukemia if matched donor is not available. CD34+ is a pan marker for human hematopoietic stem cells, including umbilical cord blood stem cell. In comparison to other sources, cord blood CD34+ cells proliferate more rapidly and produce large number of progeny cells. For ex vivo expansion of Umbilical Cord Blood- HSCs/HPCs, different combinations of cytokines have been used in many laboratories. IL2rg cytokines, including IL2, IL7 and IL15, are key cytokines in the regulation of differentiation, proliferation and survival of immune cells. IL2 is important cytokine for T cell survival and proliferation, IL7 involve in B cell development and IL15 is a key cytokine for NK cell development. In this study we evaluated the generation of T cells derived from CD34+ and CD34- cord blood mononuclear cells by using combination of cytokines including IL2, IL7 and IL15. Cultured cord blood mononuclear cells were evaluated at distinct time points during 21 days by using flow cytometry. Present study showed that differentiation of T cells derived from CD34+ cord blood mononuclear cells increased by using IL2 a...Continue Reading

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Citations

Apr 27, 2019·Reviews in the Neurosciences·Ahmad MehdipourAbbas Ebrahimi-Kalan
Mar 17, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Balal BrazvanHojjatollah Nozad Charoudeh

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