ILC2 Proliferated by IL-33 Stimulation Alleviates Acute Colitis in Rag1-/- Mouse through Promoting M2 Macrophage Polarization.

Journal of Immunology Research
Yong YouXun Sun

Abstract

This study was to identify functions of ILC2, a newly found innate lymphoid cell which mainly locates in mucosa organs like lungs and intestines, in IBD. We injected rIL-33 protein to C57/BL6 mouse to explore how IL-33 induces ILC2 proliferation. The results showed that ILC2 reached a proliferation peak at day 5 and expressed multiple surface markers like CD127, C-kit, CD69, CD44, ST2, CD27, DR3, MHCII, and CD90.2. ILC2 also expressed high quantity of IL-13 and IL-5 and few IL-17A which indicates a potentially immunological function in IBD development. Afterwards, we transferred sort purified ILC2 to Rag1-/- mouse given DSS to induce acute colitis in order to explore the innate function of ILC2. Data showed that ILC2 alleviates DSS-induced acute innate colitis by repairing epithelial barrier and restore body weight. Furthermore, we found that ILC2 can cause macrophages polarizing to M2 macrophages in the gut. Therefore, we concluded that ILC2 played a therapeutic role in mouse acute colitis.

References

Nov 1, 2007·Journal of Digestive Diseases·Franco Scaldaferri, Claudio Fiocchi
Mar 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mélanie PicheryJean-Philippe Girard
Apr 18, 2012·Inflammatory Bowel Diseases·Philipp GroβClaudia Hofmann
Jun 7, 2014·Cell·Dean YimlamaiFernando D Camargo
Sep 14, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benjamin M MattaHēth R Turnquist
Oct 29, 2014·American Journal of Respiratory and Critical Care Medicine·David J JacksonSebastian L Johnston
Apr 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonathan M HanMegan K Levings
Aug 6, 2015·Proceedings of the National Academy of Sciences of the United States of America·Laurel A MonticelliDavid Artis
Sep 15, 2015·Nature Communications·E Suzanne CohenTomas Mustelin
Sep 17, 2015·Current Opinion in Gastroenterology·Giorgos Bamias, Fabio Cominelli
Feb 26, 2016·Annual Review of Pathology·Ta-Chiang Liu, Thaddeus S Stappenbeck
Sep 28, 2016·The American Journal of Gastroenterology·Ray K BoyapatiGwo-Tzer Ho
Feb 3, 2016·Biochemistry and Biophysics Reports·Hiroko HayakawaShin-Ichi Tominaga
Nov 18, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·A M Zoltowska NilssonG P Nilsson
May 8, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jinghong LiTimothy D Bigby
May 26, 2018·International Journal of Cardiology·Alberto AimoMichele Emdin
Sep 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Loris R LopetusoTheresa T Pizarro
Oct 9, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fengge ZhuXiangmei Chen

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Methods Mentioned

BETA
Protein Purification
Assay
ELISA
PMA
flow cytometry

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