Ileal apical Na+-dependent bile acid transporter ASBT is upregulated in rats with diabetes mellitus induced by low doses of streptozotocin.

American Journal of Physiology. Gastrointestinal and Liver Physiology
Fadi AnnabaWaddah A Alrefai

Abstract

Increased intestinal bile acid absorption and expansion of the bile acid pool has been implicated in the hypercholesterolemia associated with diabetes mellitus. However, the molecular basis of the increase in bile acid absorption in diabetes mellitus is not fully understood. The ileal apical Na(+)-dependent bile acid transporter (ASBT) is primarily responsible for active reabsorption of the majority of bile acids. Current studies were designed to investigate the modulation of ASBT function and expression in streptozotocin (STZ)-induced diabetes mellitus in rats and to examine the effect of insulin on rat ASBT promoter by insulin. Diabetes mellitus was induced in Sprague-Dawley rats by intraperitoneal injection of low doses of STZ (20 mg/kg body wt) on five consecutive days. Human insulin (10 U/day) was given to a group of diabetic rats for 3 days before euthanasia. RNA and protein were extracted from mucosa isolated from the small intestine and ASBT expression was assessed by real-time quantitative RT-PCR and Western blotting. Our data showed that ASBT mRNA and protein expression were significantly elevated in diabetic rats. Insulin treatment of diabetic rats reversed the increase in ASBT protein expression to control levels. C...Continue Reading

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Citations

Mar 20, 2013·Molecular Aspects of Medicine·Tatiana Claro da SilvaPeter W Swaan
Jun 22, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alexander L TichoWaddah A Alrefai
Apr 10, 2015·American Journal of Physiology. Cell Physiology·Saminathan MuthusamyWaddah A Alrefai
Feb 20, 2020·The Journal of Biological Chemistry·Alexander L TichoWaddah A Alrefai

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