Ilimaquinone inhibits the cytotoxicities of ricin, diphtheria toxin, and other protein toxins in Vero cells

Experimental Cell Research
M P Nambiar, H C Wu

Abstract

Ilimaquinone (IQ), a metabolite from sea sponges, has been shown to cause the breakdown of Golgi membranes into small vesicular structure and to inhibit protein transport without eliciting the retrograde transport of the Golgi enzymes to the endoplasmic reticulum [P. A. Takizawa, J. K. Yucel, B. Viet, D. J. Faulkner, T. Deerinck, G. Soto, M. Ellismann, and V. Malhotra, Cell (1993) 73, 1079-1090]. We have found that incubation of Vero cells with IQ inhibited the cytotoxicity of ricin in a dose-dependent manner. The inhibition was reversed upon the removal of IQ. Neither binding and internalization of 125I-ricin nor the translocation of ricin to the cytosol was affected by IQ. However, IQ significantly inhibited the recycling and degradation of internalized 125I-ricin. Preincubation with IQ also prevented the enhancement of ricin cytotoxicity by NH4Cl or nigericin. The inhibition of ricin cytotoxicity by IQ was observed in the presence of cycloheximide, indicating that de novo protein synthesis is not required for IQ-mediated protection of Vero cells from ricin cytotoxicity. In contrast to perinuclear distribution of TRITC-labeled ricin in Vero cells, TRITC-ricin appeared in numerous small vesicles dispersed throughout the cytopl...Continue Reading

Citations

Nov 18, 2000·Infection and Immunity·X Cortes-BrattiM Thelestam
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Kirsten Sandvig, Bo van Deurs
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·A RapakS Olsnes
Sep 29, 2004·Biochimica Et Biophysica Acta·M R Hartley, J M Lord
Jun 9, 1998·Journal of Cellular Physiology·M P NambiarH C Wu
Jun 10, 2003·Journal of Applied Toxicology : JAT·Brian M Paddle
Oct 29, 2003·Toxicological Reviews·Michael J LordLynne M Roberts
Jun 15, 2013·Histochemistry and Cell Biology·Kirsten SandvigTove Irene Klokk
May 30, 2019·Natural Product Reports·Alexander E Fox RamosMehdi A Beniddir
Jun 15, 2000·The Journal of Biological Chemistry·P B Miarons, M Fresno
Feb 22, 2012·Toxins·Julien BarbierDaniel Gillet
Jul 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hilan Z KaplanJason S Kingsbury
Oct 24, 2001·The Journal of Organic Chemistry·Stéphane PoignyMohammad Samadi
Jul 9, 1999·Biochimica Et Biophysica Acta·R GhidoniA Giuliani
Aug 21, 2009·Bioorganic & Medicinal Chemistry·Byung Gyu KimMarc L Snapper

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.