ILK promotes survival and self-renewal of hypoxic MSCs via the activation of lncTCF7-Wnt pathway induced by IL-6/STAT3 signaling

Gene Therapy
Qing MaoYong-Xiang Lu

Abstract

Mesenchymal stem cells (MSCs) have been applied in treating various diseases including myocardial infarction (MI) and achieved a bit of success; however, the decreased survival rate of MSCs after transplantation greatly limited the efficacy for cell therapy. How to improve the MSC survival rate in stem cell transplantation has undoubtedly become urgent and genetic engineering may be an ideal and feasible way. In this study, we explored the effects on MSCs survival and self-renewal by overexpression of integrin-linked kinase (ILK) in MSCs under hypoxic stimulation and aimed to reveal the molecular mechanisms from the point of paracrine function of MSCs. We first found that overexpression of ILK induced the expression and secretion of IL-6 increased significantly in MSCs under hypoxic stimulation, and the survival and self-renewal of MSCs exposed to hypoxia were enhanced after ILK overexpression. Then the activation of JAK2/STAT3 signaling was detected because of the increased IL-6, and an lncRNA, named lncTCF7, was upregulated remarkably, promoting the activation of Wnt pathway that was required for keeping cell viability and stemness of MSCs. Moreover, we further verified that inhibition of STAT3 signaling by WP1066 and silenci...Continue Reading

References

Jul 28, 2004·Stem Cells·Rohan K HumphreyAlberto Hayek
Sep 5, 2006·Genes & Development·Deepak Srivastava, Sangho Yu
May 26, 2007·Circulation Research·Gregory E HanniganShoukat Dedhar
Sep 18, 2007·Stem Cell Reviews·Oliver Dreesen, Ali H Brivanlou
Jul 31, 2010·Circulation Research·Konstantinos E HatzistergosJoshua M Hare
Aug 24, 2010·Journal of Molecular and Cellular Cardiology·Maria MirotsouVictor J Dzau
Sep 21, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Arsalan ShabbirTechung Lee
Jul 6, 2012·Current Opinion in Cell Biology·Jun Qin, Chuanyue Wu
Aug 13, 2015·BMC Medicine·Naomi D'souzaMassimo Dominici
Oct 31, 2015·Journal of the American College of Cardiology·Vasileios KarantalisJoshua M Hare
Sep 13, 2017·Frontiers in Immunology·Franka LukMarcella Franquesa
Nov 18, 2017·Journal of the American College of Cardiology·Makoto NatsumedaJoshua M Hare

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
enzyme-linked immunosorbent assay
transfection
nuclear translocation
nuclear
coronary artery
PCR
electrophoresis
ELISA

Software Mentioned

BLOCK
[UNK] RNAi Designer
SPSS
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.