PMID: 2495532Apr 1, 1989Paper

Illegitimate transcription: transcription of any gene in any cell type

Proceedings of the National Academy of Sciences of the United States of America
J ChellyA Kahn

Abstract

Using in vitro amplification of cDNA by the polymerase chain reaction, we have detected spliced transcripts of various tissue-specific genes (genes for anti-Müllerian hormone, beta-globin, aldolase A, and factor VIIIc) in human nonspecific cells, such as fibroblasts, hepatoma cells, and lymphoblasts. In rats, erythroid- and liver-type pyruvate kinase transcripts were also detected in brain, lung, and muscle. The abundance of these "illegitimate" transcripts is very low; yet, their existence and the possibility of amplifying them by the cDNA polymerase chain reaction provide a powerful tool to analyze pathological transcripts of any tissue-specific gene by using any accessible cell.

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Citations

Apr 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P ReponenK Tryggvason
Nov 26, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T GionK Sugimachi
Apr 1, 1994·Molecular Reproduction and Development·T ZwingmanR P Erickson
Dec 1, 1993·Muscle & Nerve·H J KaminskiR L Ruff
Mar 29, 2002·The Journal of Pathology·Luisa ToffolattiAngelo Rosolen
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Dec 18, 2001·Seminars in Surgical Oncology·P Kienle, M Koch
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Sep 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S GoldsteinR J Shmookler Reis
Oct 1, 1995·Endocrine·C L RenderS Harvey
Jan 13, 2005·Development Genes and Evolution·Alexei Kurakin
Jun 11, 2009·Biochemical Genetics·Małgorzata TokarskaAnna M Wójcik
Apr 11, 2007·Molecular Biology Reports·Hai-Fang WangDeng-Ke Niu
Jan 1, 1993·Mutation Research·R G Cotton
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Feb 1, 1992·Genetic Analysis, Techniques and Applications·U Landegren
Mar 17, 2004·Journal of Neuroimmunology·Roxana BrunoRicardo Pujol-Borrell

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