Illuminating the Prevalence of Trypanosoma brucei s.l. in Glossina Using LAMP as a Tool for Xenomonitoring

PLoS Neglected Tropical Diseases
Lucas J CunninghamEmily R Adams

Abstract

As the reality of eliminating human African trypanosomiasis (HAT) by 2020 draws closer, the need to detect and identify the remaining areas of transmission increases. Here, we have explored the feasibility of using commercially available LAMP kits, designed to detect the Trypanozoon group of trypanosomes, as a xenomonitoring tool to screen tsetse flies for trypanosomes to be used in future epidemiological surveys. The DNA extraction method was simplified and worked with the LAMP kits to detect a single positive fly when pooled with 19 negative flies, and the absolute lowest limit of detection that the kits were able to work at was the equivalent of 0.1 trypanosome per ml. The DNA from Trypanosoma brucei brucei could be detected six days after the fly had taken a blood meal containing dead trypanosomes, and when confronted with a range of non-target species, from both laboratory-reared flies and wild-caught flies, the kits showed no evidence of cross-reacting. We have shown that it is possible to use a simplified DNA extraction method in conjunction with the pooling of tsetse flies to decrease the time it would take to screen large numbers of flies for the presence of Trypanozoon trypanosomes. The use of commercially-available L...Continue Reading

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Citations

Apr 21, 2017·PLoS Neglected Tropical Diseases·Serap AksoyJan Van Den Abbeele
Oct 18, 2018·The Onderstepoort Journal of Veterinary Research·Peter NambalaChihiro Sugimoto
Jul 9, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mariame CamaraBrice Rotureau
Oct 3, 2018·Emerging Topics in Life Sciences·Richard McCullochKeith Matthews
Sep 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tom PennanceBonnie Lee Webster
Mar 26, 2021·PLoS Neglected Tropical Diseases·Philippe Solano
Jul 10, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Herakles A GarciaMarta M G Teixeira

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Methods Mentioned

BETA
dissection
Loopamp
PCR
PCRs

Related Concepts

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African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.