Illuminating the wall: using click chemistry to image pectins in Arabidopsis cell walls.

Plant Signaling & Behavior
Charles T Anderson, Ian S Wallace

Abstract

Plant cell walls are the most abundant biomaterials on Earth and serve a multitude of purposes in human society. These complex extracellular matrices are mainly composed of polysaccharides, including cellulose, hemicelluloses, and pectins, which cannot be cytologically examined using conventional techniques. Click chemistry, which exploits a bio-orthogonal cycloaddition reaction between alkynyl and azido groups, has proven to be useful for the metabolic incorporation and detection of modified sugars in polysaccharides in animals, fungi, and bacteria, but its use to interrogate the biosynthesis or dynamics of plant cell walls has not been previously reported. Recently, we found that an alkynylated analog of fucose can be metabolically incorporated into Arabidopsis thaliana cell walls and click labeled with fluorescent probes, facilitating imaging of cell wall carbohydrates. Despite the presence of fucose in several classes of wall polysaccharides, fucose-alkyne was primarily incorporated into rhamnogalacturonan-I, a type of pectin. Using timecourse and pulse-labeling experiments, we observed the dynamics of pectin delivery and reorganization in expanding cell walls. The use of click chemistry to investigate plant cell wall archi...Continue Reading

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Citations

Feb 22, 2014·Molecular Plant·Logan BashlineYing Gu
Apr 18, 2014·Bioconjugate Chemistry·Mathias King, Alain Wagner
Jan 1, 2013·Plants·Jean-Claude MolletArnaud Lehner
Apr 10, 2020·Journal of Microscopy·A J BidhendiA Geitmann
Mar 6, 2018·Developmental Cell·Anindya GangulyRam Dixit

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