Illuminating touch deposits through cellular characterization of hand rinses and body fluids with nucleic acid fluorescence.

Forensic Science International. Genetics
Julia BurrillNunzianda Frascione

Abstract

Forensic DNA typing from touched or handled items in routine casework is increasing as the sensitivity of detection techniques improves. Our understanding of the cellular/acellular content of touch deposits and the origins of the DNA therein is still limited. This work explores the cellular content of rinses from washed and unwashed hands, as well as saliva, nasal and eye washes which could be sources of transferred DNA onto hands. Flow cytometry and microscopic examination were used to detect granularity, size and nucleic acid fluorescence data. Cellular content did not vary significantly within an individual, although some differences were observed between donors. Saliva contained populations of nucleated epithelia as well as smaller cells and debris, all positive for DNA. Hand rinses consisted almost entirely of anucleate corneocytes, many of which also stained positive for nucleic acids. These data raise questions about shed corneocyte DNA content previously assumed to be negligible.

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Citations

Dec 19, 2020·Forensic Science International. Genetics·Julia BurrillNunzianda Frascione
Jan 11, 2021·Forensic Science International. Genetics·Piyamas KanokwongnuwutAdrian Linacre
Jun 4, 2021·Electrophoresis·Julia BurrillNunzianda Frascione

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