IMACEL: A cloud-based bioimage analysis platform for morphological analysis and image classification

PloS One
Yuki ShimaharaNatsumaro Kutsuna

Abstract

Automated quantitative image analysis is essential for all fields of life science research. Although several software programs and algorithms have been developed for bioimage processing, an advanced knowledge of image processing techniques and high-performance computing resources are required to use them. Hence, we developed a cloud-based image analysis platform called IMACEL, which comprises morphological analysis and machine learning-based image classification. The unique click-based user interface of IMACEL's morphological analysis platform enables researchers with limited resources to evaluate particles rapidly and quantitatively without prior knowledge of image processing. Because all the image processing and machine learning algorithms are performed on high-performance virtual machines, users can access the same analytical environment from anywhere. A validation study of the morphological analysis and image classification of IMACEL was performed. The results indicate that this platform is an accessible and potentially powerful tool for the quantitative evaluation of bioimages that will lower the barriers to life science research.

References

Oct 1, 1986·The Journal of Cell Biology·N C Collier, M J Schlesinger
Sep 1, 1983·Molecular and Cellular Biology·L NoverD Neumann
Apr 5, 2007·Nature Protocols·Fumi Kumagai-SanoSeiichiro Hasezawa
Sep 15, 2007·Plant & Cell Physiology·Tomomi HayashiSeiichiro Hasezawa
Nov 29, 2011·Cell·Gaudenz Danuser
Aug 30, 2012·Nature Communications·Natsumaro KutsunaSeiichiro Hasezawa
Nov 22, 2013·Journal of Cell Science·Christoph Sommer, Daniel W Gerlich
Jan 7, 2017·Methods : a Companion to Methods in Enzymology·Anatole Chessel
Sep 8, 2017·Nature Communications·Philipp EulenbergF Alexander Wolf
Dec 1, 2017·BMC Bioinformatics·Curtis T RuedenKevin W Eliceiri

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Citations

Oct 15, 2021·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Kiyotaka NagakiHirotomo Takatsuka

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Methods Mentioned

BETA
fluorescence microscopy
electron microscopy
transgenic
X-ray

Software Mentioned

Bioconductor
Azure Storage SDK
ImageJ
OpenCV Azure
Mac OS X
Windows
Python
OpenCV
AlexNet
Photoshop CC

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