Image analysis assessment of the abnormal testis biopsy in male infertility

The Journal of Urology
E D KimL I Lipshultz

Abstract

We previously demonstrated that testis biopsy image analysis is an effective method for quantifying intratubular spermatogenic cells in the obstructed testis with normal spermatogenesis. As an extension of the initial report, we describe using the quantitative ploidy and morphological characteristics of cells counted with image analysis in abnormal testis biopsies obtained for a male infertility evaluation. Image analysis using a specifically designed filter was performed on Feulgen stained 5 microns, sections of paraffin embedded testicular tissue. Archival testicular tissue had been obtained using standard biopsy techniques from patients with azoospermia or severe oligospermia. Qualitative classification was based on standard evaluation of hematoxylin and eosin processed tissue. There were 62 biopsies performed in 58 men. Significant differences in the intratubular content of haploid (spermatozoa and spermatids), diploid and tetraploid cells were found among the 5 categories of abnormalities: the Sertoli-cell-only syndrome, spermatocyte arrest, spermatid arrest, hypospermatogenesis and normal spermatogenesis. Moderate variability was found in the proportion of cell types in spermatid arrest and hypospermatogenesis. Testis bio...Continue Reading

References

Apr 1, 1990·Urology·W J HellstromR W de Vere White
Aug 1, 1995·Fertility and Sterility·S Gottschalk-SabagY Sherman
Aug 24, 1995·The New England Journal of Medicine·J TesarikJ Testart
Jun 24, 1995·Lancet·S FishelS al-Hassan
Jan 1, 1997·The Journal of Urology·E D KimL I Lipshultz
May 1, 1956·Fertility and Sterility·E C ROOSEN-RUNGE
Jul 1, 1965·The Journal of Clinical Endocrinology and Metabolism·R E MANCINIJ A ANDRADA

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Citations

Jan 1, 1997·World Journal of Urology·E D Kim, L I Lipshultz
Jun 27, 2002·Current Urology Reports·R A SchoorC Niederberger
Mar 4, 2014·Environmental Toxicology and Pharmacology·Xiaozhen LiuMingyong Xie
Jun 27, 2002·Current Urology Reports·W W Lin
Dec 18, 2001·The Journal of Urology·Richard A SchoorLawrence S Ross
Jul 28, 2011·Endocrinology·Rebecca M HarrisJ Larry Jameson

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