Image analysis of hepatocyte nuclei in assessing di(2-ethylhexyl)phthalate effects eluding detection by conventional microscopy

Toxicology
D KomitowskiS Muto

Abstract

The effect of di(2-ethylhexyl)phthalate (DEHP) administered in single intraperitoneal doses of 30, 300 and 3000 mg/kg in Syrian golden hamsters was studied by means of routine pathologic investigations, electron microscopy and image analysis. The morphological evaluations did not show apparent differences between the control and treated animals. Such differences, however, were recognized by using image analysis. They concerned morphology of the hepatocyte nuclei and were defined by quantitative parameters reflecting geometrical, optical and structural properties. Of importance for differentiating dose/effect relationships were features of chromatin structure. In order to describe those features we developed special algorithms capable of identifying and characterizing regions of condensed chromatin as subimages. These were distinguished by their size, shape and optical density and showed typical distributions within the nucleus. As our results demonstrate, image analysis methods permit detection of DEHP related pathology in animals which, as far as is evident from routine morphologic evaluations, belong to the no-effect experimental group.

References

May 1, 1975·Toxicology and Applied Pharmacology·B G LakeA G Lloyd
Sep 19, 1970·Nature·R J Morris
Dec 1, 1974·Bulletin of Environmental Contamination and Toxicology·J MesD S Campbell
Aug 18, 1972·Nature·F L MayerJ L Johnson
Apr 26, 1973·The New England Journal of Medicine·T J Fagan
Jan 1, 1972·Journal of Pharmaceutical Sciences·A R SinghJ Autian
Aug 17, 1983·Biochimica Et Biophysica Acta·A E GanningG Dallner
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Zinser, D Komitowski
Oct 1, 1982·Journal of Toxicology and Environmental Health·W M KluweJ E Huff
Sep 1, 1982·Journal of Toxicology and Environmental Health·S NorthupT Schmitz

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