Image analysis of the duodenal endocrine cells in mice with particular regard to optical densitometry

Upsala Journal of Medical Sciences
M El-Salhy, J Mahdavi

Abstract

The endocrine cells in the murine proximal duodenum have been investigated by means of immunohistochemistry and computerized image analysis. Five endocrine cell types were identified, namely secretin-, gastric inhibitory polypeptide (GIP)-, gastrin-CCK-, somatostatin- and serotonin immunoreactive cells. The number of endocrine cells/mm3 epithelial cells was estimated and the cell secretory index (CSI) for different endocrine cell types was determined. Furthermore, the optical density of the cellular immunoreactivity and the immunoreactive area in the cell were determined and an index, cell immunoreactivity content was estimated as the optical density multiplied by the immunoreactive area. It has been suggested that the use of this index might better reflect the cellular peptide/amine content than does the CSI. Serotonin-immunoreactive cells were the predominant endocrine cell type, followed by gastrin/CCK-immunoreactive cells. The numbers of secretin-, GIP- and somatostatin immunoreactive cells were almost identical. All endocrine cell types were present both in crypts and in villi, but were, more numerous in the crypts, except for secretin which was more frequent in the villi.

References

Jan 1, 1989·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G S Evans
Jan 1, 1996·Scandinavian Journal of Gastroenterology·M El-Salhy, O Suhr
Mar 1, 1997·The Histochemical Journal·M el-SalhyS Zachrisson

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Citations

Jul 16, 1999·Mechanisms of Ageing and Development·O Sandström, M el-Salhy
Jul 1, 1998·Journal of Diabetes and Its Complications·M El-SalhyA Spångéus
Jun 20, 2013·Molecular Medicine Reports·Magdy El-SalhyDoris Gundersen
Aug 12, 2014·Molecular Medicine Reports·Magdy El-SalhyTrygve Hausken

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