PMID: 6748076Jun 25, 1984Paper

Image analysis of the heavy form of the acetylcholine receptor from Torpedo marmorata

Journal of Molecular Biology
F BonJ P Changeux

Abstract

The structure of the heavy (H) form of the acetylcholine receptor, which comprises two covalently linked 250,000 Mr oligomers, has been investigated by numerical analysis of electron microscope images. Na-cholate solubilized Torpedo marmorata H-form receptor was reintegrated into artificial lipid vesicles and negatively stained with uranyl acetate prior to imaging in a conventional transmission microscope. The reconstituted preparations exhibited the standard polypeptide composition of the purified receptor (alpha 2 beta gamma delta) and the same transmembrane arrangement as in the native subsynaptic membrane. Covalent disulfide linkage between the two oligomers took place exclusively through the delta chains. In agreement with previous work (Cartaud et al., 1980) the H-form appeared as "doublets" of two coplanar 9 nm rosettes at a center-to-center distance of 9.2 +/- 1.1 nm. The relative angular orientation of the two rosettes in a doublet was examined by correlation analysis in the real space. It exhibited a marked variability, few of the doublets featuring any kind of symmetry, suggesting that the two oligomers of a doublets are connected via an extended and flexible chain or loop. The area of contact between the two rosette...Continue Reading

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Citations

Jun 6, 1985·Nature·A Brisson, P N Unwin
Feb 16, 2017·British Journal of Pharmacology·Jingyi Wang, Jon Lindstrom
Nov 1, 1993·The Journal of Membrane Biology·A Devillers-ThiéryJ P Changeux
Jan 1, 1986·Annals of the New York Academy of Sciences·A KarlinN Yodh
Jul 24, 2013·The Journal of Biological Chemistry·Jean-Pierre Changeux
Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·F J Barrantes
Aug 16, 2001·Trends in Biochemical Sciences·T Grutter, J P Changeux
Dec 24, 1997·Brain Research. Brain Research Reviews·H R Arias
Jan 1, 1988·Pharmacology & Therapeutics·F J Barrantes
Sep 21, 1984·Science·J P ChangeuxP Chemouilli

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