PMID: 9548720May 23, 1998Paper

Image reconstructions of microtubules decorated with monomeric and dimeric kinesins: comparison with x-ray structure and implications for motility

The Journal of Cell Biology
A HoengerE Mandelkow

Abstract

We have decorated microtubules with monomeric and dimeric kinesin constructs, studied their structure by cryoelectron microscopy and three-dimensional image reconstruction, and compared the results with the x-ray crystal structure of monomeric and dimeric kinesin. A monomeric kinesin construct (rK354, containing only a short neck helix insufficient for coiled-coil formation) decorates microtubules with a stoichiometry of one kinesin head per tubulin subunit (alpha-beta-heterodimer). The orientation of the kinesin head (an anterograde motor) on the microtubule surface is similar to that of ncd (a retrograde motor). A longer kinesin construct (rK379) forms a dimer because of the longer neck helix forming a coiled-coil. Unexpectedly, this construct also decorates the microtubule with a stoichiometry of one head per tubulin subunit, and the orientation is similar to that of the monomeric construct. This means that the interaction with microtubules causes the two heads of a kinesin dimer to separate sufficiently so that they can bind to two different tubulin subunits. This result is in contrast to recent models and can be explained by assuming that the tubulin-kinesin interaction is antagonistic to the coiled-coil interaction within...Continue Reading

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
May 24, 1991·Science·A LupasJ Stock
Mar 1, 1986·The Journal of Cell Biology·E M MandelkowE Mandelkow
Jan 5, 1985·Journal of Molecular Biology·E M Mandelkow, E Mandelkow
Sep 20, 1985·Journal of Molecular Biology·E M MandelkowU Rühl
Dec 14, 1995·Nature·M WhittakerH L Sweeney
Jun 1, 1995·Ultramicroscopy·M WhittakerR A Milligan
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R A Walker
Jul 20, 1995·Nature·A HoengerR A Milligan
Apr 1, 1995·Journal of Muscle Research and Cell Motility·R A Cross
Feb 23, 1995·Nature·S P GilbertK A Johnson
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·D D Hackney
Jun 1, 1993·The Journal of Cell Biology·S RayJ Howard
Jan 1, 1993·Annual Review of Genetics·L S Goldstein
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y H Song, E Mandelkow
Mar 22, 1996·Journal of Molecular Biology·I M CrevelR A Cross
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Q UyedaJ A Spudich
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·C M CoppinR D Vale
Aug 2, 1996·Journal of Molecular Biology·H Sosa, R A Milligan
Jan 1, 1996·Journal of Structural Biology·D HesslerM H Ellisman
Jan 1, 1996·Journal of Structural Biology·J P Schroeter, J P Bretaudiere
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·K HiroseL A Amos
Jan 1, 1996·Annual Review of Physiology·J Howard
Oct 25, 1996·Journal of Molecular Biology·A Hoenger, R A Milligan
Oct 1, 1996·Current Biology : CB·I ArnalR H Wade
Jan 10, 1997·The Journal of Biological Chemistry·Y Z Ma, E W Taylor
Feb 1, 1997·Current Opinion in Cell Biology·L A Amos, K Hirose
Mar 21, 1997·The Journal of Biological Chemistry·W JiangD D Hackney
Feb 1, 1997·Current Biology : CB·K C Holmes

❮ Previous
Next ❯

Citations

Dec 22, 1999·Journal of Structural Biology·Y HanK H Downing
Jul 29, 1999·Biophysical Journal·E Pechatnikova, E W Taylor
Aug 31, 2001·Biophysical Chemistry·S HighsmithK Polosukhina
Feb 13, 2001·Current Opinion in Cell Biology·W R Schief, J Howard
Feb 19, 2000·Current Opinion in Cell Biology·E P Sablin
Feb 27, 1999·Current Opinion in Cell Biology·E Mandelkow, A Hoenger
Dec 26, 2001·Current Opinion in Structural Biology·E P Sablin, R J Fletterick
Jun 20, 2006·Nature Structural & Molecular Biology·Ana B AsenjoHernando Sosa
May 7, 1999·European Journal of Biochemistry·S SackE Mandelkow
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Douglas S MartinJeff Gelles
Oct 26, 1999·The Journal of Biological Chemistry·K M BrendzaW M Saxton
Apr 18, 2000·The Journal of Biological Chemistry·K M BrendzaS P Gilbert
Nov 12, 2002·The EMBO Journal·Thomas G WendtAndreas Hoenger
Jun 3, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R D ValeR Fletterick
Jun 3, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R A CrossL A Amos
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Eva-Maria Mandelkow, Eckhard Mandelkow
Oct 14, 2000·Annual Review of Cell and Developmental Biology·K H Downing
Sep 5, 2002·Annual Review of Physiology·Sharyn A Endow, Douglas S Barker
Nov 9, 2007·Journal of Cell Science·Linda A Amos, Keiko Hirose
Jun 26, 2004·Cell Structure and Function·K HiroseL A Amos
Jun 27, 2012·The Journal of Cell Biology·Katherine C RankIvan Rayment
Jun 13, 2001·The Journal of Cell Biology·J R McIntosh
Nov 22, 2000·The Journal of Cell Biology·K S ThornR D Vale
Nov 22, 2000·The Journal of Cell Biology·E W Taylor, G G Borisy
Nov 10, 2011·Journal of Structural Biology·Cédric Bouchet-MarquisAndreas Hoenger
Sep 1, 2007·Journal of Molecular Biology·Akihiro NaritaYuichiro Maéda
Oct 9, 2015·Biophysical Journal·Stephanie Guzik-LendrumSusan P Gilbert
Jun 17, 2009·Cell Motility and the Cytoskeleton·Alexander MarxEckhard Mandelkow
Apr 28, 2006·The EMBO Journal·Troy C KrzysiakAndreas Hoenger
Feb 28, 2007·Cell Motility and the Cytoskeleton·Jawdat Al-BassamRonald A Milligan
May 12, 2007·Brain Pathology·Eckhard MandelkowEva-Maria Mandelkow
Oct 17, 2012·Biology of the Cell·Katherine C Rank, Ivan Rayment
Jul 9, 2004·Journal of Molecular Biology·Montserrat Samsó, Michael P Koonce
Dec 20, 2005·Journal of Structural Biology·Dilem HizlanAndreas Hoenger
Jun 18, 2002·Journal of Structural Biology·Brian Tripet, Robert S Hodges
Aug 31, 2000·Annual Review of Biochemistry·E Nogales
May 30, 1998·Current Biology : CB·E H Egelman
Dec 23, 2009·Journal of Structural Biology·Julia CopeAndreas Hoenger
Oct 16, 2002·Structure·Huilin LiKenneth H Downing

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.