Imaging an adapted dentoalveolar complex.

Anatomy Research International
Ralf-Peter HerberSunita P Ho

Abstract

Adaptation of a rat dentoalveolar complex was illustrated using various imaging modalities. Micro-X-ray computed tomography for 3D modeling, combined with complementary techniques, including image processing, scanning electron microscopy, fluorochrome labeling, conventional histology (H&E, TRAP), and immunohistochemistry (RANKL, OPN) elucidated the dynamic nature of bone, the periodontal ligament-space, and cementum in the rat periodontium. Tomography and electron microscopy illustrated structural adaptation of calcified tissues at a higher resolution. Ongoing biomineralization was analyzed using fluorochrome labeling, and by evaluating attenuation profiles using virtual sections from 3D tomographies. Osteoclastic distribution as a function of anatomical location was illustrated by combining histology, immunohistochemistry, and tomography. While tomography and SEM provided past resorption-related events, future adaptive changes were deduced by identifying matrix biomolecules using immunohistochemistry. Thus, a dynamic picture of the dentoalveolar complex in rats was illustrated.

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Citations

Oct 17, 2013·Biotechnology Letters·Alistair BannermanLiam M Grover
Dec 12, 2012·Journal of Biomechanics·Jeremy D LinSunita P Ho

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Methods Mentioned

BETA
X-ray
light microscopy
fluorescence microscopy

Software Mentioned

XMReconstructor
Amira
Micro
Xradia 3D
Research Image Composite Editor
Image Pro Plus

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