Imaging and Characterization of Macrophage Distribution in Mouse Models of Human Prostate Cancer

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Ben T CopelandMartin G Pomper

Abstract

Prostate carcinoma consists of tumor epithelium and malignant stroma. Until recently, diagnostic and therapeutic efforts have focused exclusively on targeting characteristics of the tumor epithelium, ignoring opportunities to target inflammatory infiltrate and extracellular matrix components. Prostate tumors are rich in tumor-associated macrophages (TAMs), which can be either of the cytotoxic M1 or protumorigenic M2 phenotype. We have quantified the proportion of each in seven common human prostate tumor lines grown subcutaneously in athymic nude mice and have imaged macrophage densities in vivo in xenografts derived from these lines. A panel of seven human prostate cancer xenografts was generated in intact male athymic nude mice reflecting variable expression of the androgen receptor (AR) and prostate-specific membrane antigen (PSMA). Mice were imaged ex vivo using near-infrared fluorescence (NIRF) imaging for PSMA expression and total macrophage densities to enable direct comparison between the two. Tumors were harvested for sectioning and additional staining to delineate M1 and M2 phenotype along with vascular density. Macrophage polarization analysis of sections revealed that all xenografts were > 94% M2 phenotype, and the ...Continue Reading

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Citations

Feb 19, 2020·PLoS Biology·Robin A WelshScheherazade Sadegh-Nasseri
Feb 23, 2020·The Journal of Pathology·Tim Beltraminelli, Michele De Palma
Jun 4, 2020·Cellular Oncology (Dordrecht)·Łukasz ZadkaPiotr Dzięgiel

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