Imaging and detection technologies for image analysis in electrophoresis

Electrophoresis
K Miura

Abstract

Image capture is the first step of image analysis. There are two major devices for image capture in the field of electrophoresis. One is the charged-couple device (CCD) camera and the other is the scanner. Image capture technologies have shown great progress in recent years especially in the field of fluorescence detection and chemiluminescent detection. The direction of image analysis is high resolution, wide dynamic range and high density precision and this holds true for the CCD camera system. Various components in the CCD camera system suitable for high-sensitive fluorescence detection and chemiluminescent detection are explained. As an example, the LAS-1000plus camera system which has 1364 x 922 pixels and generates 14-bits image is introduced. Powerful cooling enables overnight exposure of chemiluminescence. Introduction of blue light-emitting diode (LED) as excitation light source improved safety to eyes. Two types of scanners for fluorescence detection and the specific characteristics are explained. There are mechanical scanning systems using confocal optics and optical scanning systems using light collecting guide optics. Deep focusing range and equal fluorescence intensity at various depth is a characteristic feature ...Continue Reading

References

Nov 1, 1990·Analytical Chemistry·S Beck, H Köster
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R TizardI Bronstein
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·I BronsteinJ C Voyta
Jan 1, 1986·Methods in Enzymology·G H Thorpe, L J Kricka
Aug 1, 1996·Analytical Biochemistry·T H SteinbergV L Singer

❮ Previous
Next ❯

Citations

Nov 16, 2002·Nucleic Acids Research·Andreas W MachlIgor Ivanov
Apr 5, 2011·The Review of Scientific Instruments·J SlobodanR J N Coope
Feb 1, 2006·Expert Review of Proteomics·Asa M Wheelock, Susumu Goto
Dec 14, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·José A Murillo PulgarínM Nieves Sánchez García
Mar 26, 2009·Electrophoresis·Ryan T Turgeon, Michael T Bowser
Sep 23, 2006·Proteomics·Ingrid MillerElisabetta Gianazza
Apr 23, 2008·Current Protocols in Protein Science·Lynn A Echan, David W Speicher
Mar 9, 2018·Current Protocols in Protein Science·Lynn A Beer, David W Speicher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.