Imaging anisotropy using differential polarization laser scanning confocal microscopy

Acta histochemica
Gábor SteinbachGyozo Garab

Abstract

We have constructed differential polarization (DP) attachments to a laser scanning microscope (LSM) for imaging the main DP quantities of anisotropic microscopic objects. The DP-LSM operates with high-frequency modulation and subsequent demodulation and displays the main DP quantities pixel by pixel. These, for linearly polarized light, include: (i) linear birefringence (LB), which is exhibited by structurally and/or optically anisotropic material; (ii) linear dichroism (LD), which carries information on the anisotropic distribution of the molecules, i.e. of their absorbance transition dipole vectors, in the sample; (iii) fluorescence-detected LD (FDLD), which carries the same information for fluorescent dyes upon excitations with two orthogonally polarized light beams; (iv) anisotropy of the fluorescence emission (r), excited with non-polarized light, which is determined by the distribution of the emission transition dipole vectors in the sample and is analogous with LD and (v) the degree of polarization of the fluorescence emission (P), excited with polarized light, which depends on the depolarization of the emission e.g. due to the rotation of molecules during their excitation lifetimes. In fluorescence regimes, the DP image...Continue Reading

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Citations

Oct 24, 2009·Photosynthesis Research·Richard CisekVirginijus Barzda
Jun 8, 2013·The Review of Scientific Instruments·Xiao WangPatrick Ferrand
Sep 12, 2013·International Journal of Molecular Sciences·Masood SamimVirginijus Barzda
Feb 10, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Aleksandar SavićKsenija Radotić
Feb 18, 2020·Physical Chemistry Chemical Physics : PCCP·Ilia M PavlovetcMasaru Kuno
Sep 15, 2020·Biomedical Optics Express·Giuseppe de VitoFrancesco Saverio Pavone
Oct 4, 2018·The Review of Scientific Instruments·Ranjan KalitaBosanta R Boruah
Mar 3, 2020·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Xiao WangJianhua Yin
Jul 25, 2021·International Journal of Molecular Sciences·Jasna Simonović RadosavljevićGábor Steinbach

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