Imaging biomarkers of inflammation in situ with functionalized quantum dots in the dextran sodium sulfate (DSS) model of mouse colitis

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
A KarwaS Murthy

Abstract

Myeloperoxidase (MPO) and proinflammatory cytokines play an important role in the development of inflammation. These markers are generally measured using tedious ELISA procedures. In this study, a novel technique utilizing antibody conjugated quantum dot nanoparticles was developed to detect Myeloperoxidase, Interleukin-1alpha (IL-1alpha) and Tumor Necrosis Factor-alpha (TNF-alpha) in vivo in the dextran sodium sulfate (DSS) model of experimental colitis. Colitis was induced in animals (n = 8 animals/group) by feeding 4% DSS solution ad libitum for seven to eight days. Quantum Dots (QDs) exhibiting fluorescence at various wavelengths were conjugated to MPO, IL-1alpha and TNF-alpha polyclonal antibodies and tested in vivo at various stages of colitis. Tissue sections obtained were imaged with confocal microscope. The image intensity obtained from the tissue specimen was correlated with clinical activity measured as Disease Activity Index (DAI). Myeloperoxidase, IL-1alpha and TNF-alpha were visualized with quantum dots on various days of disease. The intensity of quantum dots increased with the increase in inflammation. The increase in intensity showed an excellent correlation with the DAI based on the clinical parameters. The st...Continue Reading

Citations

Jun 13, 2008·Analytical and Bioanalytical Chemistry·Vasudevanpillai BijuMitsuru Ishikawa
Oct 16, 2014·Applied Physics Letters·Chao LiuD L Fan
Oct 9, 2009·Autoimmunity·Axel J HueberIain B McInnes
Mar 18, 2015·Chemical Society Reviews·K David Wegner, Niko Hildebrandt
Sep 25, 2008·The Journal of Pathology·E TholouliR J Byers
Nov 6, 2010·ACS Nano·Leonard F PeaseJeeseong Hwang

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