Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins.

Nature Methods
Walther AkemannThomas Knöpfel

Abstract

Cortical information processing relies on synaptic interactions between diverse classes of neurons with distinct electrophysiological and connection properties. Uncovering the operational principles of these elaborate circuits requires the probing of electrical activity from selected populations of defined neurons. Here we show that genetically encoded voltage-sensitive fluorescent proteins (VSFPs) provide an optical voltage report from targeted neurons in culture, acute brain slices and living mice. By expressing VSFPs in pyramidal cells of mouse somatosensory cortex, we also demonstrate that these probes can report cortical electrical responses to single sensory stimuli in vivo. These protein-based voltage probes will facilitate the analysis of cortical circuits in genetically defined cell populations and are hence a valuable addition to the optogenetic toolbox.

References

Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B Sauer, N Henderson
May 1, 1997·Nucleic Acids Research·K AkagiA Berns
Jul 17, 2001·The European Journal of Neuroscience·R SakaiT Knöpfel
Nov 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Takahiko Matsuda, Constance L Cepko
Sep 25, 2004·The Journal of Comparative Neurology·Yumiko HatanakaFujio Murakami
Oct 22, 2004·Nature Reviews. Neuroscience·Amiram Grinvald, Rina Hildesheim
Apr 12, 2005·Cell Calcium·Rosa CossartRafael Yuste
May 20, 2005·Nature·Yoshimichi MurataYasushi Okamura
Oct 6, 2005·Nature Neuroscience·Baron ChandaFrancisco Bezanilla
Jan 31, 2006·Trends in Neurosciences·Thomas KnöpfelWalther Akemann
Apr 18, 2006·Nature Neuroscience·Sylvain Crochet, Carl C H Petersen
May 25, 2006·Chemistry & Biology·Amy E PalmerRoger Y Tsien
May 10, 2007·PloS One·Dimitar DimitrovThomas Knöpfel
Dec 7, 2007·Neuron·Isabelle FerezouCarl C H Petersen
Feb 8, 2008·Cell Structure and Function·Hiroyuki KatayamaAtsushi Miyawaki
May 17, 2008·Development, Growth & Differentiation·Tomomi Shimogori, Masaharu Ogawa
May 23, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lucas Sjulson, Gero Miesenböck
Jul 16, 2008·Nature Methods·Hidekazu TsutsuiAtsushi Miyawaki
Jan 9, 2009·Frontiers in Neural Circuits·Volodymyr NikolenkoRafael Yuste
Jan 23, 2009·Nature Methods·Christoph LutzValentina Emiliani
May 20, 2009·Biophysical Journal·Walther AkemannThomas Knöpfel
Jul 23, 2009·Frontiers in Molecular Neuroscience·Amelie PerronThomas Knöpfel
Jan 13, 2010·Chemistry & Biology·Amelie PerronThomas Knöpfel
Feb 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Steven MennerickCharles F Zorumski

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Citations

Dec 29, 2012·Pflügers Archiv : European journal of physiology·Hiroki Mutoh, Thomas Knöpfel
Jul 7, 2011·ACS Chemical Neuroscience·Graham C R Ellis-Davies
Aug 17, 2012·ACS Chemical Neuroscience·Hiroki MutohThomas Knöpfel
Nov 12, 2013·Nature Biotechnology·James H Marshel, Karl Deisseroth
Dec 30, 2011·Nature Methods·Loren L Looger
Aug 13, 2010·Nature Methods·Monya Baker
Jul 28, 2013·Nature Protocols·Fumitaka Osakada, Edward M Callaway
Nov 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ping YanLeslie M Loew
Feb 9, 2013·Microscopy·Atsushi Miyawaki
May 21, 2013·Annual Review of Neuroscience·Z Josh Huang, Hongkui Zeng
Apr 13, 2013·Cell Communication and Signaling : CCS·Paul Michel Aloyse AntonyKarol Kozak
Mar 22, 2013·PloS One·Richard FairlessAdolfo Cavalié
Jul 11, 2013·PloS One·Yiyang GongMark J Schnitzer
Nov 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Thomas KnöpfelEdward S Boyden
Jul 20, 2012·Annual Review of Biomedical Engineering·Michael Levin, Claire G Stevenson
Jul 12, 2014·Annual Review of Biomedical Engineering·Melissa R WardenKarl Deisseroth
Oct 18, 2014·Cellular and Molecular Life Sciences : CMLS·Guy A Rutter, David J Hodson
Mar 5, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Emilia Entcheva
Jul 10, 2013·The Journal of Physiology·Hidekazu TsutsuiYasushi Okamura
Nov 16, 2011·The Journal of Physiology·Matthias MindererDavid J Margolis
Dec 25, 2015·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Michelle Y ChengGary K Steinberg
Jan 19, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anna DevorDavid A Boas
Feb 2, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Andy Y ShihDavid Kleinfeld
Mar 12, 2015·Biological & Pharmaceutical Bulletin·Fumitaka Osakada, Masayo Takahashi
May 15, 2015·Scientific Reports·Douglas A StoraceUhna Sung
Jul 7, 2015·Scientific Reports·Caleb R StoltzfusAleksander Rebane
Jul 23, 2013·Scientific Reports·Walther AkemannThomas Knöpfel
May 14, 2011·Developmental Neurobiology·Filippo Del Bene, Claire Wyart
Jun 21, 2013·Biology of the Cell·Eirini Papagiakoumou
Nov 7, 2012·Biology of the Cell·Spencer C AlfordThomas Knöpfel
Sep 21, 2010·Experimental Physiology·Hiroki MutohThomas Knöpfel
Apr 7, 2012·Biological Psychiatry·Yuan Mei, Feng Zhang
Feb 11, 2014·Biophysical Journal·Jennifer H HouAdam E Cohen
Mar 26, 2016·Journal of Chemical Theory and Computation·Josef MelcrPavel Jungwirth
Jun 27, 2015·Current Opinion in Chemical Biology·Thomas KnöpfelChenchen Song
Jun 5, 2015·Current Opinion in Chemical Biology·Jhon Ralph EnterinaRobert E Campbell
Jun 17, 2015·Current Opinion in Chemical Biology·François St-PierreMichael Z Lin

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