Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

Chemical Science
W J TippingA N Hulme

Abstract

Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling strategies is set to revolutionise the direct visualisation of intracellular drug uptake. Rational evaluation of a series of Raman-active labels has allowed the identification of highly active labels which have minimal perturbation on the biological efficacy of the parent drug. Drug uptake has been correlated with markers of cellular composition and cell cycle status, and mapped across intracellular structures using dual-colour and multi-modal imaging. The minimal phototoxicity and low photobleaching associated with SRS microscopy has enabled real-time imaging in live cells. These studies demonstrate the potential for SRS microscopy in the drug development process.

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Citations

Mar 21, 2019·Annual Review of Biophysics·Yihui ShenWei Min
Jul 19, 2019·Chemical Communications : Chem Comm·Jeremy SeidelWenjun Zhang
Jun 1, 2020·Journal of Biophotonics·Georgette Azemtsop MatanfackJürgen Popp
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Mar 6, 2019·Nanomaterials·Sally Vanden-HehirAlison N Hulme
Mar 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Mian WeiWei Min
Nov 5, 2020·Chemical Communications : Chem Comm·William J TippingDuncan Graham
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Feb 5, 2021·Analytical Methods : Advancing Methods and Applications·Neha ChaudharyAidan D Meade
Feb 23, 2021·Frontiers in Pharmacology·Junjie ZengShuhua Yue
Apr 7, 2021·Journal of Medicinal Chemistry·Adriana AdamczykMalgorzata Baranska
Aug 15, 2021·Proceedings of the National Academy of Sciences of the United States of America·Xingda AnBjörn M Reinhard
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Sep 11, 2021·Analytical Chemistry·Liam T WilsonNicholas C O Tomkinson
Nov 5, 2021·Chemical Communications : Chem Comm·Sian Sloan-DennisonKaren Faulds

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Methods Mentioned

BETA
imaging techniques
fluorescence microscopy
Raman

Software Mentioned

WiRE
Gaussian
FluoView FV10 - ASW
ImageJ
DFT

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