Abstract
The feasibility of ultrahigh resolution optical coherence tomography (UHR OCT) to image ex vivo and in vitro brain tissue morphology on a scale from single neuron cells to a whole animal brain was investigated using a number of animal models. Sub-2-microm axial resolution OCT in biological tissue was achieved at different central wavelengths by separately interfacing two state-of-the-art broad bandwidth light sources (titanium:sapphire, Ti:Al2O3 laser, lambdac=800 nm, Deltalambda=260 nm, Pout=50 mW and a fiber laser light source, lambdac=1350 nm, Deltalambda=470 nm, Pout=4 mW) to free-space or fiber-based OCT systems, designed for optimal performance in the appropriate wavelength regions. The ability of sub-2-microm axial resolution OCT to visualize intracellular morphology was demonstrated by imaging living ganglion cells in cultures. The feasibility of UHR OCT to image the globular structure of an entire animal brain as well as to resolve fine morphological features at various depths in it was tested by imaging a fixed honeybee brain. Possible degradation of OCT axial resolution with depth in optically dense brain tissue was examined by depositing microspheres through the blood stream to various depths in the brain of a livin...Continue Reading
References
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A GrinvaldE Bartfeld
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R A StepnoskiD Kleinfeld
Nov 22, 1991·Science·D HuangC A Puliafito
Sep 1, 1995·Nature Medicine·J G FujimotoE A Swanson
Jan 1, 1995·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·R AcharyaC Hinojosa
Jun 17, 1998·Experimental Eye Research·W DrexlerA F Fercher
May 30, 1998·Journal of Neuroscience Methods·S N RoperD H Reitze
Oct 10, 1998·Neurosurgery·S A BoppartJ G Fujimoto
Feb 13, 2001·Trends in Cognitive Sciences·R Menzel, M Giurfa
Apr 3, 2001·Nature Medicine·W DrexlerJ G Fujimoto
Apr 18, 2001·Current Opinion in Oncology·J M Hoffman
Jul 18, 2002·Physics in Medicine and Biology·A N YaroslavskyH J Schwarzmaier
Mar 27, 2003·Investigative Ophthalmology & Visual Science·Martin GloesmannWolfgang Drexler
Apr 10, 2003·Journal of Biomedical Optics·N IftimiaG J Tearney
May 14, 2003·Archives of Ophthalmology·Wolfgang DrexlerAdolf F Fercher
May 16, 2003·Optics Letters·K BizhevaH Pehamberger
Jun 21, 2003·Optics Letters·A UnterhuberA Stingl
Jul 30, 2003·Optics Letters·Mariya LazebnikStephen A Boppart
Jan 13, 2004·Journal of Biomedical Optics·Wolfgang Drexler
Aug 9, 2005·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Briain D MacNeillGuillermo J Tearney
Nov 17, 2007·Optics Letters·L VabreA C Boccara
Nov 23, 2007·Optics Letters·B PovazayE Scherzer
Nov 28, 2007·Optics Letters·I HartlR S Windeler
Dec 13, 2007·Optics Letters·W DrexlerJ G Fujimoto
Feb 15, 2008·Applied Optics·J M Schmitt, G Kumar
Jun 16, 2003·Optics Express·Yimin WangRobert Windeler
Apr 1, 1996·Journal of Biomedical Optics·A F Fercher
Citations
Apr 4, 2009·Acta neurochirurgica·H J BöhringerA Giese
Oct 28, 2011·Journal of Biomedical Optics·Khanh Quoc KieuNasser Peyghambarian
Nov 19, 2005·Journal of Biomedical Optics·M Samir JafriJoseph M Schmitt
Oct 8, 2008·Journal of Neuroscience Methods·M Samir JafriCha-Min Tang
Dec 15, 2012·Medicinal Research Reviews·Yang LiuSamuel Achilefu
Jul 17, 2010·Annals of the New York Academy of Sciences·Shailendra Joshi, Sachin Agarwal
Feb 3, 2015·IEEE Transactions on Medical Imaging·Gregory G Scott, Brittany Coats
Oct 7, 2004·Current Opinion in Neurobiology·Amit D MehtaMark J Schnitzer
Oct 9, 2013·NeuroImage·Hui WangTaner Akkin
Mar 9, 2017·Biomedical Optics Express·Olivier ThouveninA Claude Boccara
Apr 26, 2005·Journal of Biomedical Optics·Kostadinka BizhevaTuan Le
Jan 7, 2010·Optics Express·Ireneusz GrulkowskiMaciej Wojtkowski
Oct 22, 2010·Applied Optics·Manabu SatoIzumi Nishidate
Nov 20, 2012·Biomedical Optics Express·Melissa M EberleB Hyle Park
Jan 16, 2018·Bioscience Trends·Yingwei FanHongen Liao
Aug 4, 2009·Applied Optics·Manabu SatoIzumi Nishidate
Apr 17, 2012·Applied Optics·Manabu SatoIzumi Nishidate
Sep 11, 2010·Applied Optics·Manabu SatoIzumi Nishidate
Jul 3, 2015·Biomedical Optics Express·Melissa M EberleB Hyle Park
Sep 24, 2015·Optics Letters·N K ThipparapuJ K Sahu
Jan 1, 2020·BMC Medical Imaging·Xiaojun YuLinbo Liu
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Guoqiang YuTheresa M Busch
Dec 8, 2020·Biomedical Optics Express·I N DolganovaK I Zaytsev