Imaging flow dynamics in murine coronary arteries with spectral domain optical Doppler tomography.

Biomedical Optics Express
Daniel X HammerMarc D Feldman

Abstract

Blood flow in murine epicardial and intra-myocardial coronary arteries was measured in vivo with spectral domain optical Doppler tomography (SD-ODT). Videos at frame rates up to 180 fps were collected and processed to extract phase shifts associated with moving erythrocytes in the coronary arteries. Radial averaging centered on the vessel lumen provided spatial smoothing of phase noise in a single cross-sectional frame for instantaneous peak velocity measurement without distortion of the flow profile. Temporal averaging synchronized to the cardiac cycle (i.e., gating) was also performed to reduce phase noise, although resulting in lower flow profiles. The vessel angle with respect to incident imaging beam was measured with three-dimensional raster scans collected from the same region as the high speed cross-sectional scans. The variability in peak phase measurement was 10-15% from cycle to cycle on a single animal but larger for measurements among animals. The inter-subject variability is attributed to factors related to real physiological and anatomical differences, instrumentation variables, and measurement error. The measured peak instantaneous flow velocity in a ~40-µm diameter vessel was 23.5 mm/s (28 kHz Doppler phase shi...Continue Reading

References

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Dec 19, 2009·Circulation·UNKNOWN WRITING GROUP MEMBERSUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee
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Citations

Dec 11, 2013·Optics Letters·Mircea MujatHartmut Legner

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Methods Mentioned

BETA
imaging techniques

Software Mentioned

ODT
SD
OCT

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