Imaging heterogeneity of peptide delivery and binding in solid tumors using SPECT imaging and MRI

EJNMMI Research
Joost HaeckJifke Veenland

Abstract

As model system, a solid-tumor patient-derived xenograft (PDX) model characterized by high peptide receptor expression and histological tissue homogeneity was used to study radiopeptide targeting. In this solid-tumor model, high tumor uptake of targeting peptides was expected. However, in vivo SPECT images showed substantial heterogeneous radioactivity accumulation despite homogenous receptor distribution in the tumor xenografts as assessed by in vitro autoradiography. We hypothesized that delivery of peptide to the tumor cells is dictated by adequate local tumor perfusion. To study this relationship, sequential SPECT/CT and MRI were performed to assess the role of vascular functionality in radiopeptide accumulation. High-resolution SPECT and dynamic contrast-enhanced (DCE)-MRI were acquired in six mice bearing PC295 PDX tumors expressing the gastrin-releasing peptide (GRP) receptor. Two hours prior to SPECT imaging, animals received 25 MBq (111)In(DOTA-(βAla)2-JMV594) (25 pmol). Images were acquired using multipinhole SPECT/CT. Directly after SPECT imaging, MR images were acquired on a 7.0-T dedicated animal scanner. DCE-MR images were quantified using semi-quantitative and quantitative models. The DCE-MR and SPECT images were...Continue Reading

References

Oct 5, 2001·European Journal of Nuclear Medicine·D J KwekkeboomE P Krenning
Mar 1, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·David L Buckley
Jul 12, 2002·European Journal of Nuclear Medicine and Molecular Imaging·Claude ReubiBeatrice Waser
Sep 28, 2002·Journal of Magnetic Resonance Imaging : JMRI·Anwar R Padhani
Apr 30, 2003·Journal of Magnetic Resonance Imaging : JMRI·Peter L ChoykeMichael V Knopp
Aug 1, 2006·Journal of Magnetic Resonance Imaging : JMRI·Claudia WeidensteinerPeter R Allegrini
Nov 14, 2006·Cancer Imaging : the Official Publication of the International Cancer Imaging Society·Theodosia MainaStephen Mather
Apr 3, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Stephen J MatherSusan A Watson
Apr 3, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Mark T Madsen
May 1, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Dik J KwekkeboomEric P Krenning
Feb 28, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Flavio ForrerDik J Kwekkeboom
Apr 29, 2009·Methods : a Companion to Methods in Enzymology·R P J SchroederM de Jong
Oct 16, 2009·Current Medical Imaging Reviews·Thomas E Yankeelov, John C Gore
Oct 31, 2009·International Journal of Cancer. Journal International Du Cancer·Rogier P J SchroederMarion de Jong
Nov 20, 2009·IEEE Transactions on Medical Imaging·Stefan KleinJosien P W Pluim
Feb 26, 2010·European Journal of Nuclear Medicine and Molecular Imaging·Rogier P J SchroederMarion de Jong
Dec 12, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Mattias SandströmMark Lubberink
May 31, 2014·BioMed Research International·Andreas DimakakosMichael Koutsilieris
Jun 1, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ashutosh BarveKun Cheng
Nov 22, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Kristell L S ChatalicMarion de Jong
Jan 27, 2015·IEEE Transactions on Bio-medical Engineering·Majid MahrooghyDespina Kontos
Mar 21, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Simone U DalmMarleen Melis

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Citations

Jan 22, 2019·Annual Review of Food Science and Technology·Corina L ReichertJochen Weiss
Mar 27, 2021·Physics in Medicine and Biology·Abhijit J Chaudhari, Ramsey D Badawi

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BETA
imaging techniques
xenograft

Software Mentioned

Elastix
TRICKS
OptiQuant
MATLAB

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