Imaging individual proteins and nanodomains on intact cell membranes with a probe-based optical antenna

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Thomas S van ZantenMaría F Garcia-Parajo

Abstract

Optical antennas that confine and enhance electromagnetic fields in a nanometric region hold great potential for nanobioimaging and biosensing. Probe-based monopole optical antennas are fabricated to enhance fields localized to <30 nm near the antenna apex in aqueous conditions. These probes are used under appropriate excitation antenna conditions to image individual antibodies with an unprecedented resolution of 26 +/- 4 nm and virtually no surrounding background. On intact cell membranes in physiological conditions, the obtained resolution is 30 +/- 6 nm. Importantly, the method allows individual proteins to be distinguished from nanodomains and the degree of clustering to be quantified by directly measuring physical size and intensity of individual fluorescent spots. Improved antenna geometries should lead to true live cell imaging below 10-nm resolution with position accuracy in the subnanometric range.

References

Apr 20, 2002·Biophysical Journal·Russell E ThompsonWatt W Webb
Jan 8, 2004·The Journal of Cell Biology·Alessandra CambiCarl G Figdor
Dec 17, 2004·Physical Review Letters·Heinrich G FreyReinhard Guckenberger
Aug 11, 2005·Physical Review Letters·J N FarahaniB Hecht
Jan 13, 2006·Nature Chemical Biology·Anatoli IanoulJohn Paul Pezacki
Apr 12, 2006·Physical Review Letters·Pascal AngerLukas Novotny
Jul 21, 2006·Molecular Biology of the Cell·Alessandra CambiCarl G Figdor
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Aug 16, 2006·Physical Review Letters·Sergei KühnVahid Sandoghdar
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
Jan 3, 2007·Nature Cell Biology·Ken JacobsonRichard G W Anderson
Jan 5, 2007·Annual Review of Immunology·Bing-Hao LuoTimothy A Springer
May 26, 2007·Science·Stefan W Hell
Jun 20, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Bärbel I de BakkerMaria F Garcia-Parajo
Aug 7, 2007·Physical Review Letters·Lukas Novotny
Jan 31, 2008·Nano Letters·Christiane Höppener, Lukas Novotny
Jan 1, 2009·Nature Methods·Jennifer Lippincott-Schwartz, Suliana Manley
Jan 28, 2009·Nano Letters·Christiane HöppenerLukas Novotny
Oct 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thomas S van ZantenMaria F Garcia-Parajo

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Citations

Dec 7, 2011·Annals of Biomedical Engineering·Christopher V Kelly, Harold G Craighead
May 9, 2012·Quarterly Reviews of Biophysics·Christiane Höppener, Lukas Novotny
Oct 14, 2011·Accounts of Chemical Research·Peter HinterdorferYves F Dufrêne
Oct 19, 2011·ACS Chemical Biology·Alessandra Cambi, Diane S Lidke
Oct 27, 2012·Nano Letters·Mathieu MivelleMaria F Garcia-Parajo
Oct 16, 2013·Nano Letters·Lars NeumannNiek F van Hulst
Jul 3, 2013·Physical Chemistry Chemical Physics : PCCP·Toshinori MotegiKei Murakoshi
Jan 20, 2011·Biophysical Journal·Carlo ManzoMaria F Garcia-Parajo
Sep 16, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Daniel M CzajkowskyZhifeng Shao
Dec 3, 2011·Chemical Society Reviews·Xin Ting Zheng, Chang Ming Li
Dec 25, 2015·Optics Express·Yuanyuan ChenReuven Gordon
Feb 27, 2016·F1000Research·Thomas S van Zanten, Satyajit Mayor
Feb 7, 2017·Reports on Progress in Physics·Francesco Monticone, Andrea Alù
Apr 2, 2018·The Journal of Chemical Physics·Martín CaldarolaMichel Orrit
Mar 10, 2017·Light, Science & Applications·Maria PrincipeAndrea Cusano
Apr 9, 2014·Microscopy Research and Technique·Mathieu MivelleMaria F Garcia-Parajo
Sep 9, 2021·Biochemical Society Transactions·Pamina M Winkler, María F García-Parajo
Sep 8, 2021·Chemical Reviews·Chunghwan JungJunsuk Rho

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