Imaging macropinosomes during Shigella infections

Methods : a Companion to Methods in Enzymology
Sonja KühnJost Enninga

Abstract

Macropinocytosis is the uptake of extracellular fluid within vesicles of varying size that takes place during numerous cellular processes in a large variety of cells. A growing number of pathogens, including viruses, parasites, and bacteria are known to induce macropinocytosis during their entry into targeted host cells. We have recently discovered that the human enteroinvasive, bacterial pathogen Shigella causes in situ macropinosome formation during its entry into epithelial cells. These infection-associated macropinosomes are not generated to ingest the bacteria, but are instead involved in Shigella's intracellular niche formation. They make contacts with the phagocytosed shigellae to promote vacuolar membrane rupture and their cytosolic release. Here, we provide an overview of the different imaging approaches that are currently used to analyze macropinocytosis during infectious processes with a focus on Shigella entry. We detail the advantages and disadvantages of genetically encoded reporters as well as chemical probes to trace fluid phase uptake. In addition, we report how such reporters can be combined with ultrastructural approaches for correlative light electron microscopy either in thin sections or within large volume...Continue Reading

References

Mar 23, 2005·Nature Methods·Lars KuerschnerChristoph Thiele
Jan 13, 2006·Nature Chemical Biology·Jennifer A Prescher, Carolyn R Bertozzi
Mar 24, 2006·Nature Methods·Tor Erik Rusten, Harald Stenmark
Feb 23, 2008·Chemistry & Biology·Arnaud GautierKai Johnsson
Jan 16, 2009·Angewandte Chemie·Anne B Neef, Carsten Schultz
Apr 7, 2010·Nature Chemical Biology·Devaraj SubramanianCarsten Schultz
Jun 11, 2011·Cellular Microbiology·Cristiane de Souza CarvalhoMaximiliano Gabriel Gutierrez
Sep 20, 2011·Trends in Biotechnology·Georgeta Crivat, Justin W Taraska
Jan 16, 2013·Methods in Cell Biology·Alexander A Mironov, Galina V Beznoussenko
Aug 5, 2016·Communicative & Integrative Biology·Andreas Jeschke, Albert Haas
Sep 16, 2016·Biology of the Cell·Andrey Bolbat, Carsten Schultz

❮ Previous
Next ❯

Citations

Oct 27, 2018·Nanoscale Research Letters·Parisa Foroozandeh, Azlan Abdul Aziz
Jan 8, 2020·International Journal of Molecular Sciences·Alex N FrickensteinLacey R McNally
Jan 19, 2021·Expert Opinion on Drug Delivery·Mohammad HajimolaaliFarid Dorkoosh
Apr 14, 2021·Cellular Microbiology·Yuen-Yan ChangVirginie Stévenin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.