Imaging mass spectrometry-guided fast identification of antifungal secondary metabolites from Penicillium polonicum

Applied Microbiology and Biotechnology
Jing BaiYuquan Xu

Abstract

The discovery of antibiotics from microorganisms using classic bioactivity screens suffers from heavy labor and high re-discovery rate. Recently, largely uncovered biosynthetic potentials were unveiled by new approaches, such as genetic manipulation of "silent" biosynthetic gene clusters, innovative data acquisition, and processing methods. In this work, a fast and efficient antibiotic identification pipeline based on the MALDI-TOF imaging mass spectrometry was applied to study the antifungal metabolites during the confrontation of two fungal species, Penicillium polonicum and wilt-inducing fungus Fusarium oxysporum. By visualizing the spatial distribution of metabolites directly on the microbial colony and surrounding media, we predicted the antifungal candidates before isolating pure compounds and individually testing their bioactivity, which subsequently guided the identification of target molecules using classic chromatographic methods. Via this procedure, we successfully identified two antifungal metabolites, fructigenine A and B, which belong to indole alkaloid class and were not reported for antifungal activity. Our work assigned new bioactivity to previously reported compounds and more importantly showed the efficiency ...Continue Reading

References

Jul 23, 2002·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Atsumi ShimadaYasuo Kimura
Apr 28, 2006·FEMS Microbiology Letters·Jae Hyung KoAhn Joong-Hoon
Aug 5, 2009·Chemical & Pharmaceutical Bulletin·Lin DuQianqun Gu
Nov 17, 2009·Nature Chemical Biology·Yu-Liang YangPieter C Dorrestein
Oct 11, 2011·Nature Chemical Biology·Roland D KerstenPieter C Dorrestein
Jul 24, 2012·Journal of Bacteriology·Jane Y YangPieter C Dorrestein
Sep 26, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tanja Thorskov BladtThomas Ostenfeld Larsen
Sep 3, 2014·Natural Product Reports·Wei XuYi Tang
Dec 6, 2014·Journal of Natural Products·Souvik KusariMichael Spiteller
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Rachel BleichElizabeth A Shank
Jun 30, 2015·Nature Reviews. Microbiology·Peter J Rutledge, Gregory L Challis
Mar 10, 2016·Proteomics·Matthias HolzlechnerMartina Marchetti-Deschmann
Oct 19, 2016·Current Opinion in Microbiology·Mark S ButlerMatthew A Cooper
May 11, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Ying-Ning HoYu-Liang Yang
Jun 13, 2017·Nature Chemical Biology·Kenneth D ClevengerNeil L Kelleher
Apr 11, 2016·Mitochondrial DNA. Part B, Resources·Xincong KangBingyan Xie
Mar 1, 2003·The New Phytologist·D FravelC Alabouvette

❮ Previous
Next ❯

Citations

Jan 11, 2020·Acta Crystallographica. Section C, Structural Chemistry·Xue Jie TanJian Ping Ma
Oct 6, 2020·Natural Product Research·Xue-Yun CaiZhong-Tao Ding

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR

Software Mentioned

FlexImaging

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.