Abstract
The discovery of antibiotics from microorganisms using classic bioactivity screens suffers from heavy labor and high re-discovery rate. Recently, largely uncovered biosynthetic potentials were unveiled by new approaches, such as genetic manipulation of "silent" biosynthetic gene clusters, innovative data acquisition, and processing methods. In this work, a fast and efficient antibiotic identification pipeline based on the MALDI-TOF imaging mass spectrometry was applied to study the antifungal metabolites during the confrontation of two fungal species, Penicillium polonicum and wilt-inducing fungus Fusarium oxysporum. By visualizing the spatial distribution of metabolites directly on the microbial colony and surrounding media, we predicted the antifungal candidates before isolating pure compounds and individually testing their bioactivity, which subsequently guided the identification of target molecules using classic chromatographic methods. Via this procedure, we successfully identified two antifungal metabolites, fructigenine A and B, which belong to indole alkaloid class and were not reported for antifungal activity. Our work assigned new bioactivity to previously reported compounds and more importantly showed the efficiency ...Continue Reading
References
Jul 23, 2002·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Atsumi ShimadaYasuo Kimura
Apr 28, 2006·FEMS Microbiology Letters·Jae Hyung KoAhn Joong-Hoon
Aug 5, 2009·Chemical & Pharmaceutical Bulletin·Lin DuQianqun Gu
Nov 17, 2009·Nature Chemical Biology·Yu-Liang YangPieter C Dorrestein
Dec 22, 2009·Natural Product Reports·Shu-Ming Li
May 17, 2011·Angewandte Chemie·Yu-Liang YangPieter C Dorrestein
Oct 11, 2011·Nature Chemical Biology·Roland D KerstenPieter C Dorrestein
May 23, 2012·Marine Drugs·Yun-Jing ChaiWei Hua
May 30, 2012·Journal of Proteomics·David J GonzalezPieter C Dorrestein
Jul 24, 2012·Journal of Bacteriology·Jane Y YangPieter C Dorrestein
Aug 22, 2013·MBio·Matthew F TraxlerRoberto Kolter
Sep 26, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tanja Thorskov BladtThomas Ostenfeld Larsen
Dec 18, 2013·Journal of Industrial Microbiology & Biotechnology·Junko YaegashiClay C C Wang
Sep 3, 2014·Natural Product Reports·Wei XuYi Tang
Dec 3, 2014·Phytochemistry·Herana Kamal SeneviratneNorman G Lewis
Dec 6, 2014·Journal of Natural Products·Souvik KusariMichael Spiteller
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Rachel BleichElizabeth A Shank
Mar 19, 2015·MBio·Charlotte F MichelsenPeter Stougaard
Jun 30, 2015·Nature Reviews. Microbiology·Peter J Rutledge, Gregory L Challis
Aug 6, 2015·The Journal of Antibiotics·Ying-Yue BuMichio Namikoshi
Mar 5, 2016·The ISME Journal·Joseph E SprakerNancy P Keller
Mar 10, 2016·Proteomics·Matthias HolzlechnerMartina Marchetti-Deschmann
Oct 19, 2016·Current Opinion in Microbiology·Mark S ButlerMatthew A Cooper
Jul 26, 2016·Frontiers in Microbiology·Talwinder KaurRajesh K Manhas
Apr 4, 2017·Nature Microbiology·Jens Christian NielsenJens Nielsen
May 11, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Ying-Ning HoYu-Liang Yang
Jun 13, 2017·Nature Chemical Biology·Kenneth D ClevengerNeil L Kelleher
Sep 19, 2017·Cell Chemical Biology·B Christopher HoeflerPaul D Straight
Apr 11, 2016·Mitochondrial DNA. Part B, Resources·Xincong KangBingyan Xie
Mar 1, 2003·The New Phytologist·D FravelC Alabouvette
Citations
Jan 27, 2019·Expert Review of Molecular Diagnostics·Thomas J Walsh, Matthew W McCarthy
Jan 11, 2020·Acta Crystallographica. Section C, Structural Chemistry·Xue Jie TanJian Ping Ma
Mar 7, 2019·Frontiers in Microbiology·Sonja L KnowlesNicholas H Oberlies
Oct 6, 2020·Natural Product Research·Xue-Yun CaiZhong-Tao Ding