Imaging mass spectrometry of gold nanoparticles in a tissue section as an immunohistochemical staining mass probe

Journal of Mass Spectrometry : JMS
Daiki MukoYasuro Niidome

Abstract

For analysis of low abundance peptides in a tissue section, immunohistochemical staining through antibody-antigen interaction is a usual technique. The antibody is conjugated with a probe moiety that aids in highly sensitive detection. Gold nanoparticles, which show excellent chemical stability and variation of surface modifications, are expected to act as a sensitive mass probe to desorb gold ions (Au+ , Au2+ , Au3+ ) that are distinguishable from fragment ions from organic molecules. Here, green fluorescent proteins (GFP) in a tissue section of a transgenic zebrafish were detected by the gold mass probe conjugated with antibodies. Due to the efficient ionization and desorption of gold ions, imaging mass spectrometry of Au2+ ions indicated the distribution of gold nanoparticles stained in a tissue section, and the mass signal distribution was consistent with the area where the GFP-expressing cells were distributed. Conventional immunofluorescence techniques showed intense autofluorescence that come from intrinsic fluorophores in the tissue section. In contrast, the gold nanoparticles acted as an immunostaining mass probe that displayed significantly lower background signals.

References

Nov 5, 2003·Journal of Mass Spectrometry : JMS·Michelle L ReyzerRichard M Caprioli
Jan 19, 2006·Current Opinion in Chemical Biology·Alan Waggoner
Apr 7, 2010·Analytical Biochemistry·Shaleen ViraPaul S Blank
Jul 21, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Liane WehderFerdinand von Eggeling
Apr 2, 2014·Journal of Proteomics·Megan M GesselRichard M Caprioli
Jan 27, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Michaela Aichler, Axel Walch

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