Imaging mass spectrometry reveals unique protein profiles during embryo implantation.

Endocrinology
Kristin E BurnumRichard M Caprioli

Abstract

A reciprocal interaction between the implantation-competent blastocyst and receptive uterus is an absolute requirement for implantation, a process crucial for pregnancy success. A comprehensive understanding of this interaction has yet to be realized. One major difficulty in clearly defining this discourse is the complexity of the implantation process involving heterogeneous cell types of both the uterus and blastocyst, each endowed with unique molecular signatures that show dynamic changes during the course of pregnancy. Whereas gene expression studies by in situ hybridization or immunohistochemistry have shown differential expression patterns of specific genes during implantation, there is no report how numerous signaling proteins are spatially displayed at specific times and stages of implantation in the context of blastocyst-uterine juxtaposition. Using in situ imaging (matrix assisted laser desorption/ionization) mass spectrometry directly on uterine sections, here we provide molecular composition, relative abundance, and spatial distribution of a large number of proteins during the periimplantation period. This approach has allowed us for the first time to generate in situ proteome profiles of implantation and interimplan...Continue Reading

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Citations

Jul 12, 2008·Histochemistry and Cell Biology·Axel WalchHeinz Höfler
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·M Lisa ManierRichard M Caprioli
Jun 23, 2011·Analytical Chemistry·Tyler A ZimmermanEdwin De Pauw
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Apr 29, 2010·Chemical Reviews·Kamila Chughtai, Ron M A Heeren
Dec 12, 2012·Nature Medicine·Jeeyeon ChaSudhansu K Dey
Sep 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erin H Seeley, Richard M Caprioli
Aug 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Cheng-Chih HsuPieter C Dorrestein
Jun 3, 2011·The Journal of Biological Chemistry·Erin H SeeleyRichard M Caprioli
Oct 2, 2013·Human Reproduction Update·Signe AltmäeAndres Salumets
Jul 16, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Katie L MeehanAndrew N Stephens
Feb 13, 2009·Expert Review of Proteomics·Mahesh ChoolaniSinuhe Hahn
Jul 6, 2012·Trends in Biotechnology·Rima Ait-BelkacemDaniel Lafitte
May 10, 2011·Journal of Proteomics·William M HardestyRichard M Caprioli
Feb 5, 2011·Trends in Biotechnology·Erin H Seeley, Richard M Caprioli
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Jan 9, 2020·Nature Communications·Paul D PiehowskiKristin E Burnum-Johnson

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