Imaging membrane lipid order in whole, living vertebrate organisms.

Biophysical Journal
Dylan M OwenKatharina Gaus

Abstract

We report the first imaging of membrane lipid order in a whole, living vertebrate organism. This was achieved with the phase-sensitive, membrane-partitioning probe Laurdan in conjunction with multiphoton microscopy to image cell membranes in various tissues of live zebrafish embryos in three dimensions, including hindbrain, retina, muscle, gut, and kidney. The data also allowed quantitative analysis of membrane order, which showed high lipid order in the apical surfaces of polarized epithelial cells. The transition of membrane order imaging from cultured cell lines to living organisms is an important step forward in understanding the physiological relevance of membrane microdomains including lipid rafts.

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Citations

Dec 14, 2011·Nature Protocols·Dylan M OwenKatharina Gaus
Oct 5, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marisa ZallocchiDominic Cosgrove
Jan 7, 2016·Frontiers in Plant Science·Xiaoyu ZhaoYinglang Wan
Jun 15, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Dylan M OwenKatharina Gaus
Jul 27, 2010·Biophysical Journal·Saame Raza Shaikh
Sep 29, 2011·Chemistry and Physics of Lipids·Laura CantùAlessandro Prinetti
Jul 22, 2014·Molecular Membrane Biology·Joanna M KwiatekKatharina Gaus
Jan 21, 2012·Biophysical Journal·Larisa YurlovaMikael Simons
Jun 4, 2013·Current Protocols in Cell Biology·Richard K P Benninger, David W Piston
Apr 30, 2020·Laboratory animal research·Ahmed Abu-Siniyeh, Walid Al-Zyoud
Jun 4, 2018·Cancer Metastasis Reviews·Alfredo Erazo-OliverasRobert S Chapkin
Jun 2, 2020·APL Bioengineering·Jagadish Sankaran, Thorsten Wohland
Sep 15, 2020·Frontiers in Cellular and Infection Microbiology·Jacques AugenstreichCatherine Astarie-Dequeker
Jun 18, 2020·Scientific Reports·Thomas SchubertWinfried Römer
Nov 16, 2013·Plant Physiology·Patricia Gerbeau-PissotFrançoise Simon-Plas

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