Imaging membrane potential changes from dendritic spines using computer-generated holography

Neurophotonics
Dimitrii TaneseD Zecevic

Abstract

Electrical properties of neuronal processes are extraordinarily complex, dynamic, and, in the general case, impossible to predict in the absence of detailed measurements. To obtain such a measurement one would, ideally, like to be able to monitor electrical subthreshold events as they travel from synapses on distal dendrites and summate at particular locations to initiate action potentials. It is now possible to carry out these measurements at the scale of individual dendritic spines using voltage imaging. In these measurements, the voltage-sensitive probes can be thought of as transmembrane voltmeters with a linear scale, which directly monitor electrical signals. Grinvald et al. were important early contributors to the methodology of voltage imaging, and they pioneered some of its significant results. We combined voltage imaging and glutamate uncaging using computer-generated holography. The results demonstrated that patterned illumination, by reducing the surface area of illuminated membrane, reduces photodynamic damage. Additionally, region-specific illumination practically eliminated the contamination of optical signals from individual spines by the scattered light from the parent dendrite. Finally, patterned illumination ...Continue Reading

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Citations

Nov 23, 2017·Journal of Biophotonics·Luiza FilipisMarco Canepari
Oct 20, 2018·Biomedical Optics Express·Peter QuickeAmanda J Foust
Sep 11, 2019·Optics Express·Miguel Carbonell-LealOmel Mendoza-Yero
Jan 9, 2019·Frontiers in Cellular Neuroscience·Emiliano RonzittiEirini Papagiakoumou
Jan 24, 2019·Frontiers in Cellular Neuroscience·Kenichi MiyazakiWilliam N Ross
Oct 9, 2019·Nature Methods·Filippo PisanoFerruccio Pisanello
Jan 1, 2021·Frontiers in Cellular Neuroscience·Kevin DorgansMarylka Yoe Uusisaari
Jan 28, 2021·Journal of the American Chemical Society·Monica A GonzalezEvan W Miller
May 7, 2021·Science Advances·Miao-Ping ChienAdam E Cohen

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