Imaging of amyloid-β in Alzheimer's disease transgenic mouse brains with ToF-SIMS using immunoliposomes

Biointerphases
Louise CarlredPeter Sjövall

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has been proven to successfully image different kinds of molecules, especially a variety of lipids, in biological samples. Proteins, however, are difficult to detect as specific entities with this method due to extensive fragmentation. To circumvent this issue, the authors present in this work a method developed for detection of proteins using antibody-conjugated liposomes, so called immunoliposomes, which are able to bind to the specific protein of interest. In combination with the capability of ToF-SIMS to detect native lipids in tissue samples, this method opens up the opportunity to analyze many different biomolecules, both lipids and proteins, at the same time, with high spatial resolution. The method has been applied to detect and image the distribution of amyloid-β (Aβ), a biologically relevant peptide in Alzheimer's disease (AD), in transgenic mouse brain tissue. To ensure specific binding, the immunoliposome binding was verified on a model surface using quartz crystal microbalance with dissipation monitoring. The immunoliposome binding was also investigated on tissue sections with fluorescence microscopy, and compared with conventional immunohistochemistry using...Continue Reading

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Citations

May 19, 2017·Analytical and Bioanalytical Chemistry·Kaija SchaepeJürgen Janek
May 29, 2018·Journal of Neurochemistry·Mandana HunterMaurice A Curtis
Oct 14, 2020·Journal of Neurochemistry·Martina Blank, Carsten Hopf
Aug 5, 2021·Metabolic Brain Disease·Laura K SchnackenbergE Ellen Jones

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