Abstract
The Fas death receptor interacts with its ligand FasL and induces apoptosis. The Fas-FasL interaction occurs at the cell-cell interface in vivo, since both proteins are expressed in cell membranes. However, most studies on the Fas signal pathway have been performed in a nonphysiological manner by using soluble molecules (antibody or crosslinked FasL proteins) to stimulate Fas. The Fas-FasL interaction at the cell-cell contact site has only been studied recently, but the information derived from cell-cell interaction studies is still rather limited and not necessarily consistent with the past results obtained by using soluble Fas-stimulatory molecules. Therefore, we develop a novel reconstituted system that mimics the Fas-FasL interaction at cell-cell contact sites for further examination of the physiological Fas-FasL signaling system. By conjugating FasL extracellular domains to planar lipid bilayers, we created a model cell membrane to activate Fas-expressing cells. Using this system, we generated an image of Fas-FasL interactions at the cell-membrane interface at high resolution. We observed that the Fas-FasL interaction between two membranes creates submicron membrane microdomains. Shortly after microdomain formation, the ce...Continue Reading
References
Feb 7, 1997·Cell·S Nagata
Feb 28, 1998·The Journal of Biological Chemistry·M MuzioV M Dixit
May 2, 1998·The EMBO Journal·C ScaffidiM E Peter
May 23, 1998·The Journal of Experimental Medicine·P SchneiderJ Tschopp
Sep 4, 1998·Cell·H LiJ Yuan
Dec 12, 2001·Molecular and Cellular Biology·Alicia Algeciras-SchimnichMarcus E Peter
Jan 31, 2003·Molecular and Cellular Biology·Nils HollerJürg Tschopp
Mar 7, 2003·Molecular Cell·Mrudula DonepudiMarkus G Grütter
Aug 4, 2004·The Journal of Cell Biology·Sebastian KreuzHarald Wajant
Mar 30, 2005·The Journal of Cell Biology·Frank HenklerHarald Wajant
Feb 25, 2006·The EMBO Journal·Kyeong-Hee LeeAndrew C Chan
May 8, 2007·Cell·Marcus E PeterDavid H Lynch
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yoshihisa KaizukaRonald D Vale
Feb 26, 2009·Immunity·Andreas StrasserShigekazu Nagata
Apr 29, 2009·The Journal of Cell Biology·Yoshihisa KaizukaRonald D Vale
May 25, 2010·Nature Structural & Molecular Biology·Jay T Groves, John Kuriyan
Citations
Oct 21, 2015·FEBS Letters·M Florencia SánchezDolores C Carrer
Jan 27, 2015·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Jun LiaoTeng Xu
Oct 11, 2016·Journal of Molecular Biology·Andrew K LewisJonathan N Sachs
Oct 23, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Ming YanYan Xia
Dec 25, 2013·Journal of Agricultural and Food Chemistry·Huan RaoHong-Yan Li