Imaging of Fas-FasL membrane microdomains during apoptosis in a reconstituted cell-cell junction

Biochemical and Biophysical Research Communications
Ling ZhangNobutaka Hanagata

Abstract

The Fas death receptor interacts with its ligand FasL and induces apoptosis. The Fas-FasL interaction occurs at the cell-cell interface in vivo, since both proteins are expressed in cell membranes. However, most studies on the Fas signal pathway have been performed in a nonphysiological manner by using soluble molecules (antibody or crosslinked FasL proteins) to stimulate Fas. The Fas-FasL interaction at the cell-cell contact site has only been studied recently, but the information derived from cell-cell interaction studies is still rather limited and not necessarily consistent with the past results obtained by using soluble Fas-stimulatory molecules. Therefore, we develop a novel reconstituted system that mimics the Fas-FasL interaction at cell-cell contact sites for further examination of the physiological Fas-FasL signaling system. By conjugating FasL extracellular domains to planar lipid bilayers, we created a model cell membrane to activate Fas-expressing cells. Using this system, we generated an image of Fas-FasL interactions at the cell-membrane interface at high resolution. We observed that the Fas-FasL interaction between two membranes creates submicron membrane microdomains. Shortly after microdomain formation, the ce...Continue Reading

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Citations

Oct 21, 2015·FEBS Letters·M Florencia SánchezDolores C Carrer
Jan 27, 2015·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Jun LiaoTeng Xu
Oct 11, 2016·Journal of Molecular Biology·Andrew K LewisJonathan N Sachs
Oct 23, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Ming YanYan Xia

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