Imaging of human sodium-iodide symporter gene expression mediated by recombinant adenovirus in skeletal muscle of living rats

European Journal of Nuclear Medicine and Molecular Imaging
Hyun Suk YangSeong-Wook Park

Abstract

We evaluated the feasibility of non-invasive imaging of recombinant adenovirus-mediated human sodium-iodide symporter (hNIS) gene expression by (99m)TcO(4)(-) scintigraphy in skeletal muscle of rats. Replication-defective recombinant adenovirus encoding hNIS gene [Rad-CMV-hNIS 5x10(7), 2x10(8) or 1x10(9) plaque forming units (pfu)] or beta-galactosidase gene (Rad-CMV-LacZ 1x10(9) pfu) was injected into the right biceps femoris muscle of rats ( n=5-6 for each group). Three days after gene transfer, scintigraphy was performed using a gamma camera 30 min after injection of (99m)TcO(4)(-) (1.85 MBq). An additional two rats injected with 1x10(9) pfu of Rad-CMV-hNIS underwent (99m)TcO(4)(-) scintigraphy with sodium perchlorate. After the imaging studies, rats were sacrificed for assessment of the biodistribution of (99m)TcO(4)(-) and measurement of hNIS mRNA expression. In all the rats injected with 1x10(9) pfu of Rad-CMV-hNIS, hNIS expression was successfully imaged by (99m)TcO(4)(-) scintigraphy, while rats injected with Rad-CMV-LacZ or lower doses of Rad-CMV-hNIS failed to show uptake. The biodistribution studies indicated that a significantly different amount of (99m)TcO(4)(-) was retained in the liver ( p<0.001) and the right mu...Continue Reading

Citations

May 11, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Matthias W KronFlorian Kreppel
Sep 13, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Andreas WortmannFlorian Kreppel
Apr 14, 2010·Thyroid : Official Journal of the American Thyroid Association·Philippe R FrankenThierry Pourcher
Oct 9, 2007·Nuclear Medicine and Biology·Inna SerganovaRonald Blasberg
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May 14, 2021·Molecular Therapy Oncolytics·Susanna C ConcilioKah-Whye Peng

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