Imaging of Mouse Brain Fixated in Ethanol in Micro-CT

BioMed Research International
Jana MrzílkováPetr Zach

Abstract

Micro-CT imaging is a well-established morphological method for the visualization of animal models. We used ethanol fixation of the mouse brains to perform high-resolution micro-CT scans showing in great details brain grey and white matters. It was possible to identify more than 50 neuroanatomical structures on the 5 selected coronal sections. Among white matter structures, we identified fornix, medial lemniscus, crossed tectospinal pathway, mammillothalamic tract, and the sensory root of the trigeminal ganglion. Among grey matter structures, we identified basal nuclei, habenular complex, thalamic nuclei, amygdala, subparts of hippocampal formation, superior colliculi, Edinger-Westphal nucleus, and others. We suggest that micro-CT of the mouse brain could be used for neurohistological lesions evaluation as an alternative to classical neurohistology because it does not destroy brain tissue.

References

Jun 26, 2010·Medical Image Analysis·Martin BaikerBoudewijn P F Lelieveldt
Sep 13, 2012·Physics in Medicine and Biology·Hongkai WangArion F Chatziioannou
Sep 24, 2013·Journal of Neuroscience Methods·Sahar GhanavatiJohn G Sled
Jan 21, 2014·Connective Tissue Research·Gili R S NavehSteve Weiner
Jun 26, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Jan BuytaertJoris Dirckx
Jul 12, 2014·Stroke; a Journal of Cerebral Circulation·Jin-Yong ParkDong-Eog Kim
Jul 18, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Christoph JudFranz Pfeiffer

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Methods Mentioned

BETA
X-ray
chips
optical microscopy
dissection

Software Mentioned

Pixelman
Volex
Allen Mouse Atlas
CTVox
Adult Mouse Brain Atlas Brain Library
Xnview

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